tailieunhanh - Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands. | Research Article Turk J Agric For 34 (2010) 145-154 © TÜBİTAK doi: Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae İlyas BOZLAĞAN1, Abdurrahman AYVAZ2, Fatma ÖZTÜRK2, Leyla AÇIK3, Mikail AKBULUT2, Semih YILMAZ1 1 Graduate School of Natural and Applied Sciences, Erciyes University, Kayseri - TURKEY 2Department of Biology, Faculty of Arts and Sciences, Erciyes University, 38039, Kayseri - TURKEY 3Department of Biology, Faculty of Arts and Science, Gazi University, 06500, Ankara - TURKEY Received: Abstract: Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands. In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL–1 was used. LC99 values for isolates I36, I37, and I40 were , , and for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were and for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others. Key words: Bacillus thuringiensis, cry1 primer, Ephestia kuehniella, Plodia interpunctella Tarım alanlarından izole edilen Bacillus thuringiensis izolatlarındaki cry1 geninin belirlenmesi ve bu izolatların iki farklı depo zararlısı güve .