tailieunhanh - Genetic identification of clones and the genetic structure of seed crops in a Pinus brutia seed orchard
Megagametophyte tissue in seeds from a Pinus bruia Ten. seed orchard was analyzed for 9 enzyme systems (aconitase, alcohol dehydrogenase, glutamate dehydrogenase, glutamate oxaloacetate-transaminase, malate dehydrogenase, menadione reductase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, and shikimate dehydrogenase) encoded by 14 loci using starch gel electrophoresis. | Research Article Turk J Agric For 34 (2010) 127-134 © TÜBİTAK doi: Genetic identification of clones and the genetic structure of seed crops in a Pinus brutia seed orchard Nuray KAYA*, Kani IŞIK Biology Department, Faculty of Arts and Sciences, Akdeniz University, 07058 Antalya - TURKEY Received: 2009 Abstract: Megagametophyte tissue in seeds from a Pinus bruia Ten. seed orchard was analyzed for 9 enzyme systems (aconitase, alcohol dehydrogenase, glutamate dehydrogenase, glutamate oxaloacetate-transaminase, malate dehydrogenase, menadione reductase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, and shikimate dehydrogenase) encoded by 14 loci using starch gel electrophoresis. Twenty-eight clones and 3 ramets per clone were sampled from the orchard for analysis. Isozyme patterns of the clones clearly indicate that each clone had a different genotype when overall loci were taken into consideration. In 5 of the 28 clones, 1 of the 3 ramets did not match the other 2 ramets at 2 or more loci. This indicates that about 6% of the studied ramets in the seed orchard were mislabeled during and/or before being planted in the orchard. The Adh2-1 allele was unique to clone ; therefore, such an allele could be used to determine the extent to which a specific clone contributes to the seed crop. Of the 14 loci studied, 7 (50%) were polymorphic ( criterion). Overall, 28 alleles were observed in the orchard trees and seed crops. Three of the 28 alleles (allele 2 at Aco, allele 2 at Got1, and allele 3 at Pgi2) were not observed in the orchard clones, but were observed only in the orchard seed crops, which indicates that these alleles originated from outside pollen sources. Important allelic differences were noted between the gene pool of the orchard clones and the gene pool of the orchard seed crops, most probably due to the presence of these alien alleles in the orchard crops. We also observed that there was , , and .
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