tailieunhanh - Determination of γ aminobutyric acid (gaba) in rambutan fruit cv. rongrian by hplc elsd and separation of gaba from rambutan fruit using dowex 50w x8 column

Numerous studies have been carried out to investigate the biological properties exhibited by rambutan, such as antimicrobial property, antioxidant property, anti-hyperglycemic potential, antiHerpes Simplex virus activity, cytotoxic activity, antiinflammatory activity and antidiabetic activity,. Invite you to consult the documentation. | Journal of Chromatographic Science, 2016, Vol. 54, No. 3, 445–452 doi: Advance Access Publication Date: 20 November 2015 Article Article Determination of γ-Aminobutyric Acid (GABA) in Rambutan Fruit cv. Rongrian by HPLC-ELSD and Separation of GABA from Rambutan Fruit Using Dowex 50W-X8 Column Maneerat Meeploy* and Rujira Deewatthanawong Agricultural Technology Department, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3, Tambon Khlong Ha, Amphoe Khlong Luang, Changwat Pathum Thani 12120, Thailand *Author to whom correspondence should be addressed. Email: maneerat@ Received 5 May 2015; Revised 14 September 2015 Abstract A high-performance liquid chromatography method coupled with an evaporative light scattering detector (ELSD) was validated for the determination of γ-aminobutyric acid (GABA) in rambutan fruit without any sample pretreatment or derivatization. In the concentration range of – mg/mL GABA, the ELSD response was linear with a correlation coefficient (r) >. Limit of detection and limit of quantitation were found to be and µg/mL, respectively. The method enabled the complete separation of GABA in the aqueous extract of rambutan flesh from the impurity peaks at min. The recoveries of sample added GABA were obtained in the range of –. Intraday and interday relative standard deviations were . The range of the analytical method was obtained during the linearity and accuracy studies. The precision data used for this assessment were the precision of the analysis of five injections at each amount of added GABA in the accuracy study. The acceptable range was defined as the concentration interval over which linearity, accuracy and precision were obtained with acceptance criteria. LOD and LOQ LOD and LOQ for GABA were calculated from the linearity data using residual standard deviation (SD) of the regression lines and Table I. The Gradient Elution Program for .