tailieunhanh - Genome-wide identification and analysis of growth regulating factor genes in Brachypodium distachyon: In silico approaches

Growth-regulating factor (GRF) genes may play important roles for regulating growth and development in different plant tissues and organs. Here we report the first genome-wide analysis of the GRF gene family in Brachypodium. | Turkish Journal of Biology Turk J Biol (2014) 38: 296-306 © TÜBİTAK doi: Research Article Genome-wide identification and analysis of growth regulating factor genes in Brachypodium distachyon: in silico approaches 1, 2 3 Ertuğrul FİLİZ *, İbrahim KOÇ , Hüseyin TOMBULOĞLU Department of Crop and Animal Production, Çilimli Vocational School, Düzce University, Çilimli, Düzce, Turkey 2 Department of Molecular Biology and Genetics, Faculty of Science, Gebze Institute of Technology, Gebze, Kocaeli, Turkey 3 Department of Biology, Faculty of Science and Arts, Fatih University, Büyükçekmece, İstanbul, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Growth-regulating factor (GRF) genes may play important roles for regulating growth and development in different plant tissues and organs. Here we report the first genome-wide analysis of the GRF gene family in Brachypodium. We performed in silico comparative analysis of GRF genes, including their structure, duplication in the genome, conserved motifs, and phylogenetic relationship. At the end of the study, 10 BdGRF genes were identified. The highest number of GRF genes was identified on chromosome 1 with 5 members, whereas the least number of genes (only 1 member) was found on chromosomes 2, 4, and 5. Of those, a single segmental duplication was observed in the Brachypodium genome. Average exon and intron numbers were determined as 3 and 4, respectively. Motif analysis showed that WRC and QLQ residues were consistent in all GRF protein sequences. Gene Ontology terms showed that 10 BdGRF proteins grouped in the same biological function, biological process, and cellular component groups. In addition, we compared the new BdGRF proteins with the other monocot and dicot GRF proteins sequences. Phylogenetic analysis revealed that GRF proteins of monocot and dicot species were clustered together in a .