tailieunhanh - The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: A genetic approach

In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. | Turkish Journal of Biology Turk J Biol (2014) 38: 48-62 © TÜBİTAK doi: Research Article The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach 1 2 3 4 5, 2 Osman EROL , Hilal Betül KAYA , Levent ŞIK , Metin TUNA , Levent CAN *, Muhammed Bahattin TANYOLAÇ 1 Department of Botany, Faculty of Science, İstanbul University, İstanbul, Turkey 2 Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, İzmir, Turkey 3 Department of Biology, Faculty of Arts and Sciences, Celal Bayar University, Manisa, Turkey 4 Department of Field Crops, Faculty of Agriculture, Namık Kemal University, Tekirdağ, Turkey 5 Department of Biology, Faculty of Arts and Sciences, Namık Kemal University, Tekirdağ, Turkey Received: Accepted: Published Online: Printed: Abstract: In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from pg in C. asumaniae to pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from (M-CAA/E-AGC) to (M-CAT/E-AAG) and showed an average of . In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, .