tailieunhanh - Compounds from culture broth of marine bacterium Micromonospora sp. (G047)

Five compounds L-phenyl alanine (1), 1-methyl-6-acetyl-α-D-glucopyranoside (2), thymine (3), uracil (4), and (2R,3S)-butane-2,3-diol (5) were isolated and characterized from the culture broth of the marine bacterium Micromospora sp. (G047 strain), which was isolated from the sediment collecting from the coast of Co To – Quang Ninh. The structures of all isolates were determined by spectroscopic analysis including MS and 2D NMR, as well as by comparison with reported data in the literature. Compound 2 has been previously synthesized, however, this is the first its report from a natural source. | Vietnam Journal of Chemistry, International Edition, 55(2): 207-210, 2017 DOI: Compounds from culture broth of marine bacterium Micromonospora sp. (G047) Vu Van Nam, Doan Thi Mai Huong*, Chau Van Minh, Pham Van Cuong Institute of Marine Biochemistry, Vietnam Academy of Science and Technology Received 16 September 2016; Accepted for publication 11 April 2017 Abstract Five compounds L-phenyl alanine (1), 1-methyl-6-acetyl-α-D-glucopyranoside (2), thymine (3), uracil (4), and (2R,3S)-butane-2,3-diol (5) were isolated and characterized from the culture broth of the marine bacterium Micromospora sp. (G047 strain), which was isolated from the sediment collecting from the coast of Co To – Quang Ninh. The structures of all isolates were determined by spectroscopic analysis including MS and 2D NMR, as well as by comparison with reported data in the literature. Compound 2 has been previously synthesized, however, this is the first its report from a natural source. Keywords. Micromospora sp., marine bacterium, 1-methyl-6-acetyl-α-D-glucopyranoside, thymine, uracil, L-phenyl alanine. 1. INTRODUCTION Actinomycetes are aerobic, spore forming grampositive bacteria, belonging to the order Actinomycetales characterized with substrate and aerial mycelium growth. They are rich source of secondary metabolites with diverse biological activity [1]. Being a large group of microbial resources of wide practical use and high commercial value, actinomycetes contribute to around 70 % of the source of antibiotics and also produce numerous non-antibiotic bioactive metabolites, such as enzymes, enzyme inhibitors, immunological regulators, anti-oxidation reagents, and so on [2]. Actinomycetes are widely distributed in natural habitats, especially soil and ocean. Among the marine microorganisms actinomycetes comprise an important group. They have tremendous potential to synthesize bioactive secondary metabolites [3]. In this paper, we report the isolation .