tailieunhanh - Silver nanoparticles: Cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. | Turkish Journal of Biology Turk J Biol (2013) 37: 573-581 © TÜBİTAK doi: Research Article Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells 1 2,3, 4 3,5 6 Hakan ÇİFTÇİ , Mustafa TÜRK *, Uğur TAMER , Siyami KARAHAN , Yusuf MENEMEN Department of Chemistry and Chemical Processing Technologies, Kırıkkale Vocational High School, Kırıkkale University, Yahşihan, Kırıkkale, Turkey 2 Department of Bioengineering, Faculty of Engineering, Kırıkkale University, Yahşihan, Kırıkkale, Turkey 3 Kırıkkale University Scientific and Research Laboratories, Yahşihan, Kırıkkale, Turkey 4 Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkey 5 Department of Histology and Embryology, Faculty of Veterinary Medicine, Kırıkkale University, Yahşihan, Kırıkkale, Turkey 6 Department of Biology, Faculty of Sciences and Arts, Kırıkkale University, Yahşihan, Kırıkkale, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-VFLUOS immunostaining and double staining of Hoechst dye with propidium iodide. In AFM and TEM analyses, the sizes of AgNPs varied from 16 nm to 20 nm. AgNPs were 80 nm in hydrodynamic diameter with a zeta potential of – mV. The WST-1 assay resulted in an IC50 value of 40

• Sinh học
• Atomic force microscopy
• Transmission electron microscopy
• Silver nanoparticles
• Hydroxylamine hydrochloride
• Plasmonic photothermal therapy