tailieunhanh - Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. | Turkish Journal of Biology Turk J Biol (2013) 37: 530-537 © TÜBİTAK doi: Research Article Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea 1 1, 2 3 1 Kutsal KESİCİ , İnci TÜNEY *, Doğuş ZEREN , Mustafa GÜDEN , Atakan SUKATAR 1 Department of Biology, Faculty of Science, Ege University, Bornova, İzmir, Turkey 2 Department of Materials Science and Engineering, İzmir Institute of Technology, Urla, İzmir, Turkey 3 Department of Mechanical Engineering, Faculty of Engineering, İzmir Institute of Technology, Urla, İzmir, Turkey Received: Accepted: Published Online: Printed: Abstract: Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms. Key words: .
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