tailieunhanh - Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın
The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. | Turkish Journal of Biology Turk J Biol (2013) 37: 514-519 © TÜBİTAK doi: Research Article Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın 1,2, 1 1 2,3 Erman ORYAŞIN *, Hacı Halil BIYIK , Gamze BAŞBÜLBÜL , Bülent BOZDOĞAN Department of Biology, Faculty of Arts and Sciences, Adnan Menderes University, Aydın, Turkey 2 Science and Technology Research and Application Center, Adnan Menderes University, Aydın, Turkey 3 Department of Medical Microbiology, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined by polymerase chain reaction. The transferability of resistance was tested by conjugation. A total of 57 enterococci were isolated. All erythromycin-resistant enterococci had ermB and all tetracycline-resistant isolates had tetM genes. Macrolide and tetracycline resistances were transferable by conjugation to Enterococcus faecalis JH2-2. Transconjugants were confirmed by pulsed-field gel electrophoresis analysis. Our study showed the existence of .
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