tailieunhanh - Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 479-490 © TÜBİTAK doi: Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers Emel USLU*, Gözde Damla ERTUĞRUL, Mehmet Tekin BABAÇ Department of Biology, Faculty of Science and Literature, Abant İzzet Baysal University, Bolu, Turkey Received: Accepted: Published Online: Printed: Abstract: Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300–3500 bp with RAPD and 125–3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard’s coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were between T. hirtum All. and T. constantinapolitanum Ser. for RAPD and between T. medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa. Key words: Trifolium, Fabaceae, Bolu, Turkey, RAPD, SSR, genetic relationship 1. Introduction The clover genus, Trifolium L., belongs to one of the largest groups in the family Fabaceae. It contains about 255 species (Zohary and Heller, 1984; Gillet