tailieunhanh - Comparison of methodologies for the identification of aroma compounds in strawberry

Three strawberry (Fragaria x ananassa. Duch.) varieties commercially grown in Israel (Tamar, Yael and Malach) were studied for their volatile compositions. Two techniques were compared: headspace solid phase micro extraction (HS-SPME) and liquid-liquid extraction (organic solvent: tert-butyl methyl ether) by gas chromatography/mass spectrometry (GC/MS). | Turk J Agric For 29 (2005) 383-390 © TÜB‹TAK Comparison of Methodologies for the Identification of Aroma Compounds in Strawberry Ebru KAFKAS, Salih KAFKAS University of Çukurova, Faculty of Agriculture, Department of Horticulture, 01330, Balcali, Adana - TURKEY Margery KOCH-DEAN Department of Vegetable Crops, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan 50250, ISRAEL Wilfried SCHWAB Lehrstuhl fur Lebensmittelchemice, Universitat Wurzburg, Am Hubland, D-97074, Wurzburg, GERMANY Biomolecular Food Technology, TU Munich, Lise-Meitner-Str. 34, 85354 Freising, GERMANY Olga LARKOV, Noa LAVID, Einat BAR, Uzi RAVID, Efraim LEWINSOHN* Department of Vegetable Crops, Agricultural Research Organization (ARO), Newe Ya’ar Research Center, . Box 1021, Ramat Yishay 30-095, ISRAEL Received: Abstract: Three strawberry (Fragaria x ananassa. Duch.) varieties commercially grown in Israel (Tamar, Yael and Malach) were studied for their volatile compositions. Two techniques were compared: headspace solid phase micro extraction (HS-SPME) and liquid-liquid extraction (organic solvent: tert-butyl methyl ether) by gas chromatography/mass spectrometry (GC/MS). The influences of techniques on the volatile compounds were tested by comparing the volatiles determined in the 3 varieties. Malach, the most aromatic variety, accumulates high levels of furanones and esters compared to the other varieties. Differences in the aroma profiles obtained by utiliszing different techniques were noted. HS-SPME was more suitable for the determination of very volatile and nonpolar esters, while liquid extraction was more appropriate for the determination of the polar and less volatile furanones. Key Words: Strawberry, volatiles, aroma, SPME, GC/MS Çilekte Aroma Maddelerinin Tan›mlanmas›nda Kullan›lan Metodlar›n Karfl›laflt›r›lmas› Özet: Bu çal›flmada ‹srail’de ticari olarak yetifltiricili¤i yap›lan (Fragaria x ananassa. Duch) 3 çilek çeflidinde (“Tamar”, “Yael” .