tailieunhanh - Total protein content and SDS page in pear scions grafted on quince a and pear seedling rootstocks

This study was conducted to investigate the effects of various rootstocks on the total soluble protein content of pear scions, and to detect a polypeptide as a marker that would be associated with pear/quince compatibility/incompatibility. | Turk J Agric For 29 (2005) 91-96 © TÜB‹TAK Total Protein Content and SDS-PAGE in Pear Scions Grafted on Quince A and Pear Seedling Rootstocks Hatice GÜLEN* Uluda¤ University, Faculty of Agriculture, Department of Horticulture, Görükle Campus, 16059, Bursa - TURKEY Ali KÜDEN Çukurova University, Faculty of Agriculture, Department of Horticulture, 01330, Adana - TURKEY Joseph POSTMAN USDA-ARS, National Clonal Germplasm Repository, Corvallis, OR 97333 - USA Rajeev ARORA 4139 Horticulture Hall, Iowa State University, Ames, IA 50011-1100 - USA Received: Abstract: This study was conducted to investigate the effects of various rootstocks on the total soluble protein content of pear scions, and to detect a polypeptide as a marker that would be associated with pear/quince compatibility/incompatibility. Bark samples were collected from 5 year-old pear scions grafted on QA and pear seedling rootstocks (PS). Total soluble protein contents of bark tissues of 4 pear scions, Passa Crassana (PC), Beurre Hardy (BH), Beurre Bosc (BB) and Bartlett (BT), were determined using the Bradford assay. Protein profiles of bark tissues taken from the graft combinations were determined using SDS-PAGE. In general, the scions grafted on QA had a greater total protein content than those on PS rootstock. The highest protein content was determined in BH/QA combination. However, no linear correlation was detected between total protein content and graft compatibility. In SDSPAGE, the protein profiles of the scions were similar. However, a 63 kDa protein band determined in compatible pear scions (PC and BH) was faintly observed in an intermediate compatible scion (BB), but was not detected in the incompatible scion (BT). Our results indicated that this polypeptide could be associated with pear/quince graft compatibility/incompatibility. Key Words: Graft incompatibility, pear, quince, protein, SDS-PAGE Quince A ve Armut Çö¤ür Anaçlar›na Afl›l› Armut Çeflitlerinde Toplam Protein .

• Nông nghiệp
• Graft incompatibility
• Pear seedling rootstocks
• Compatible pear scions
• Total soluble protein
• Quince compatibility
• Graft compatibility
• Fruit crops
• Horticultural crops
• Graft incompatibility in horticultural crops
• Grafted cucumber
• Grafted pumpkin
• Pith cavity
• Grafted seedling production
• Quince Cydonia oblanga
• Pear Pyrus communis
• Woody plants
• Polyacrylamide gel electrophoresis
• Graft compatibility characteristics