tailieunhanh - Exploration of genotype specific fingerprinting of Nigella sativa L. using RAPD markers

Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. Th is investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers. | M. S. IQBAL, S. NADEEM, S. MEHBOOB, A. GHAFOOR, M. I. RAJOKA, A. S. QURESHI, B. NIAZ Research Article Turk J Agric For 35 (2011) 569-578 © TÜBİTAK doi: Exploration of genotype specific fingerprinting of Nigella sativa L. using RAPD markers Muhammad Sajjad IQBAL1,2,5,*, Shahid NADEEM2, Shahid MEHBOOB2, Abdul GHAFOOR3, Muhammad Ibrahim RAJOKA2, Afsari Sharif QURESHI1, Bushra NIAZ4 1 Genetics Laboratory, Department of Biochemistry, Quaid-I-Azam University, Islamabad, 46000 - PAKISTAN 2 Department of Bioinformatics, GC University, Allama Iqbal Road, 38000, Faisalabad - PAKISTAN 3 Institute of Agri-Biotechnology & Genetic Resources, National Agricultural Research Center, Islamabad, 45500 - PAKISTAN 4 Department of Post-Harvest, Ayub Agriculture Research Institute, Faisalabad - PAKISTAN 5 Department of Genetics, Hazara University, Mansehra, 21300 - PAKISTAN Received: Abstract: Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. This investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers. From 58 random primers used, 15 primers generated 249 reproducible and scorable amplification products across all the genotypes, out of which 164 (66%) fragments were polymorphic revealing a high level of polymorphism among these genotypes. The proportion of common bands was low (34%). The size of the amplification products on agarose gels ranged between and kb. In 13 genotypes, 27 bands of different masses (kilobases) were recorded and were considered specific to those genotypes. These specific/amplified PCR products can be used as molecular markers for identification of germplasm and resource protection of Nigella sativa L. genotypes. Specific bands were observed for individual primers that could resolve genetic diversity among several genotypes (PK-020545, PK-020567,

• Nông nghiệp
• Genetic relatedness
• Genotype specific bands
• Random priming
• Quantitative traits loci
• Sequence characterized amplified region
• Amplified fragment length polymorphism
• Samba mahsuri
• Complete panicle emergence
• Morphological traits
• Molecular characterization
• Complete panicle emergence mutant lines
• BMC Genomics
• Transmission networks
• Outbreaks investigations
• RNA viruses
• BMC Bioinformatics
• Copy number variation
• CNV calling methods
• Monozygotic twins
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• Bacterial genomes
• Phylogenetic analysis
• Distance matrix
• Large scale bacterial sequencing
• Genetic relationships
• Journal of Zoology
• Context dependent aggression
• Intraspecific aggression
• Social structure
• Dear enemy effect
• Nasty neighbor effect
• DNA barcoding
• Vibrio cholerae
• Whole genome sequencing
• Multilocus variable number tandem repeat analysis
• Outbreak surveillance
• Household transmission