tailieunhanh - L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 887-897 © TÜBİTAK doi: L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture Virginia SARROPOULOU*, Kortessa DIMASSI-THERIOU, Ioannis THERIOS Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece Received: Accepted: Published Online: Printed: Abstract: In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L–1 IBA plus mg L–1 L-arginine and 1 mg L–1 IBA plus 1 mg L–1 L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L–1 IBA alone or combined with 1 mg L–1 L-arginine. In the Gisela 6 explants, 2 mg L–1 IBA without L-arginine significantly enhanced root number () and root fresh and dry weight, as well as rooting percentage (100%). Root length was longest (38 mm) in the combination of the lowest IBA ( mg L–1) and highest L-arginine (2 mg L–1) concentrations. Thus, L-arginine promotes the positive effect of IBA on rooting with regard to both root number and root length in both cherry rootstocks. In the CAB-6P explants, L-arginine combined with IBA had an inhibitory effect on leaf chlorophyll content, whereas in the Gisela 6 rootstock it had absolutely no effect. In the CAB-6P rootstock, depleted levels of proline in roots were observed, showing the osmoregulation and osmotic adjustment mechanisms. The carbohydrate concentration in leaves was greatest with mg L–1 IBA plus mg L–1 L-arginine, and .