tailieunhanh - L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 887-897 © TÜBİTAK doi: L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture Virginia SARROPOULOU*, Kortessa DIMASSI-THERIOU, Ioannis THERIOS Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece Received: Accepted: Published Online: Printed: Abstract: In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L–1 IBA plus mg L–1 L-arginine and 1 mg L–1 IBA plus 1 mg L–1 L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L–1 IBA alone or combined with 1 mg L–1 L-arginine. In the Gisela 6 explants, 2 mg L–1 IBA without L-arginine significantly enhanced root number () and root fresh and dry weight, as well as rooting percentage (100%). Root length was longest (38 mm) in the combination of the lowest IBA ( mg L–1) and highest L-arginine (2 mg L–1) concentrations. Thus, L-arginine promotes the positive effect of IBA on rooting with regard to both root number and root length in both cherry rootstocks. In the CAB-6P explants, L-arginine combined with IBA had an inhibitory effect on leaf chlorophyll content, whereas in the Gisela 6 rootstock it had absolutely no effect. In the CAB-6P rootstock, depleted levels of proline in roots were observed, showing the osmoregulation and osmotic adjustment mechanisms. The carbohydrate concentration in leaves was greatest with mg L–1 IBA plus mg L–1 L-arginine, and .

• Nông nghiệp
• Cherry rootstock rooting
• Amino acids
• Plant tissue culture
• Biochemical characteristics
• Tissue culture
• Advances in tissue culture
• Tissue culture of cucurbits
• Strategies for tissue culture
• Propagation in cucurbits
• plant tissue culture practice
• tissue culture technology
• tissue engineering applications
• plant tissue
• microorganisms
• introduction to animal cell culture
• Types of tissue culture
• Cell culture
• Primary explant culture
• Organ culture
• Plant tissue and cell culture
• Cell culture in Vietnam
• Forty five years of development
• Plant tissue in Vietnam
• Plant Propagation
• The Background
• Plant Tissue Culture Procedure
• Developmental Biology
• Somatic Embryogenesis
• Clonal progagation of Vietnamese
• Orthosiphon stamineus benth
• Sinensetin using plant tissue culture technique
• Plant tissue culture technique
• Accession containing high content
• Tissue culture embryo
• Mature embryos
• Evaluation and validation of rice
• Rice (Oryza sativa L
• Genotypes for tissue culture response
• Pharmaceutical compounds
• Secondary metabolism
• Value pharmaceutical compounds
• Plant tissue culture technology
• Secondary hardened
• Tissue culture plantlets
• Secondary hardened tissue culture plantlets
• Growth and yield of banana
• Banana as influenced by age
• Low cost medium
• MS medium
• Cost effectiveness
• Conventional tissue culture media
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• Hydrangea macrophylla
• In vitro
• Large scales of hydrangea macrophylla
• Tissue culture technique
• Hydrangea macrophylla using tissue culture technique
• Plant density
• Ney pooovan
• Tissue culture banana
• Growth tissue culture banana
• planting density on growth
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• Cell culture insert
• Clip on scaffold
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• Endangered plant species
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• Review on plant tissue culture
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• Potato (Solanum tuberosum L )
• Tissue culture of potato
• In vitro micro propagation
• Culture medium
• Prunus dulcis
• Tissue culture medium
• Nas hypothesis
• Micropropagation medium
• Indole 3 butyric acid
• Culture Media
• Emerging Economy
• Shoot Regeneration
• Abiotic Stress Conditions
• BMC Biotechnology
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• BMC Musculoskeletal Disorders
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• Blood culture bottles
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• Callus induction
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• Banana tissue culture propagated plantlets
• Clonal fidelity
• Assessment of clonal fidelity
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• Banana (Musa spp ) plant
• BMC Plant Biology
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• Dwarfing genes
• Small up regulated response
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