tailieunhanh - The novel methods of development of the maintainer and cytoplasmic male sterile lines with different cytoplasms based on chromosome single-segment substitution lines in rice

The present study was carried out with the objective of developing new parental lines of hybrid rice based on chromosome single-segment substitution lines (SSSLs), on which the restorer and maintainer genes Rf3 (rf3) and Rf4 (rf4) were harbored in rice. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 441-446 © TÜBİTAK doi: The novel methods of development of the maintainer and cytoplasmic male sterile lines with different cytoplasms based on chromosome single-segment substitution lines in rice 1,2 1, Jian CAI , Guiquan ZHANG * School of Life Science, Fu Yang Teachers College, Fu Yang, Anhui, . China 2 The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, . China 1 Received: Accepted: Published Online: Printed: Abstract: The present study was carried out with the objective of developing new parental lines of hybrid rice based on chromosome single-segment substitution lines (SSSLs), on which the restorer and maintainer genes Rf3 (rf3) and Rf4 (rf4) were harbored in rice. To develop the new 3-line hybrid, the SSSL W23-19-06-06-11 with the genotype Rf3Rf3/Rf4Rf4 [a strong restorer line for wild abortive (WA), dwarf-wild abortive (DA), and Yegong (Y) cytoplasmic male sterile (CMS) lines] was crossed 4 times as the male parent with 4 CMS lines: 2 typical WA-type CMS lines of Bobai A and Zhenshan 97A, 1 typical Y-type CMS line of Y-Huanong A, and 1 typical DA-type CMS line of Xieqingzao A. In the BC3F2 populations, the male sterile plants (MSPs) with WA-, Y-, and DA-type CMS sources and nuclear background of W23-19-06-06-11 and their maintainer plants (MPs) were developed by backcrossing and marker-assisted selection. All the MSPs showed 100% pollen and spikelet sterility and the panicle length ranged from 20 to cm. The extent of incomplete panicle exertion in the entries ranged from – to –. The pollen grain viability assay with I2KI of the MSPs revealed 100% pollen sterility, consisting of both typical and spherical abortive pollen grains. The MPs showed normal

• Nông nghiệp
• Three line hybrid rice
• Chromosome single segment substitution line
• Development of restorer line
• Maintainer line
• Molecular marker assisted selection
• BMC Genomics
• The Jinchuan yak
• Population genetics
• Plateau adaptation
• Molecular breeding
• Markerassisted selection