tailieunhanh - PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey

Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 457-467 © TÜBİTAK doi: PCR detection of Fusarium oxysporum f. sp. radicis-lycopersici and races of F. oxysporum f. sp. lycopersici of tomato in protected tomato-growing areas of the eastern Mediterranean region of Turkey 1, 2 Ayşegül ÇOLAK *, Mehmet BİÇİCİ Biological Control Research Station, Adana, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Çukurova University, Adana, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Fusarium crown and root rot (caused by Fusarium oxysporum f. sp. radicis-lycopersici, FORL) and Fusarium wilt (caused by Fusarium oxysporum f. sp. lycopersici, FOL) are the most important diseases to affect tomatoes in protected growing conditions in the eastern Mediterranean region of Turkey. These diseases cause significant yield losses in this region. Polymerase chain reaction (PCR) studies were used to characterize 87 isolates from Adana and Mersin provinces, representative of different locations. Among them, 60% and 40% of the plants were determined to have FORL and FOL, respectively. FORL and FOL race 3 are the dominant pathogens in this region. Some differences between morphological identification and molecular detection (PCR) were observed. Key words: Fusarium oxysporum f. sp. lycopersici, Fusarium oxysporum f. sp. radicis-lycopersici, PCR detection, tomato 1. Introduction In covered tomato-growing areas in the eastern Mediterranean region, it was previously known that the wilt disease caused by Fusarium oxysporum f. sp. lycopersici (Sacc.) . Snyder & . Hans (FOL) was of prime importance. Later, crown and root-rot diseases caused by F. oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (FORL) were encountered more frequently (Yücel and Çınar 1989; Can et al. 2004). F. oxysporum has spread .

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• Fusarium oxysporum f
• sp
• lycopersici
• Fusarium oxysporum f
• sp
• radicis lycopersici
• PCR detection
• Tomato growing
• Molecular detection
• Prevalence and molecular detection
• Blood protozoa
• Domestic pigeon
• Haemoprotozoan infection
• Brucella spp
• Molecular detection of Brucella spp
• Reproductive disorders
• Vaginal mucus
• Brucella abortus
• Real time PCR
• Molecular detection of Brucella abortus
• Buffaloes in Gujarat
• Japanese encephalitis
• Pigs and punjab
• Serological and molecular detection
• Molecular detection of japanese encephalitis
• Naturally infected pigs
• Molecular protocol
• Establishing a molecular protocol
• Detection of EGFR mutations
• Non small cell lung cancer
• The molecular protocol constructed
• Multidrug resistant
• Molecular detection of Rifampicin
• Molecular detection of Isoniazid
• Mycobacterium Tuberculosis complex
• Using line probe assay
• Molecular detection of brucella organism
• Brucella organism
• Brucella organism from milk
• Milk products
• Ehrlichia canis
• Nested PCR
• Canine monocytic ehrlichiosis
• Molecular detection of canine monocytic ehrlichiosis
• Pathomorphological and molecular detection
• Black spot
• Asperisporium caricae
• Incited byasperisporium caricae
• Detection and molecular characterization
• Virusfree garlic
• Bulb associated potyviruses in garlic
• Field response
• Molecular detection of bulb associated potyviruses
• Garlic through molecular diagnosis
• BMC Veterinary Research
• Pig brucellosis
• Molecular evidence
• Systematically quantify and identify
• Molecular techniques
• Listeria monocytogenes
• Antibiotic sensitivity
• Antibiotic sensitivity profile
• Cattle sources of aizawl
• Ziehl Neelsen stain
• Rapid molecular detection
• Mycobacterium tuberculosis
• Comparison to other conventional methods
• Conventional methods
• Polymerase chain reaction assays
• Brucella abortus from cattle
• Brucella abortus from buffalo
• DNA extraction
• Major streptococcus spp
• Antimicrobial resistance
• Bovine mastitis
• Antimicrobial resistance pattern
• Vibrio alginolyticus
• Isothermal amplification
• Recombinase polymerase amplification
• Lateral flow dipstick
• Clinico seroepidemiology
• Molecular characterization of brucellosis
• Brucellosis in animals
• Brucella antibodies by RBPT
• Chili pepper
• Molecular marker
• Resistant chili pepper cultivars
• Anthracnose resistance associated molecular markers
• Resistant chili pepper cultivars in Vietnam
• Cinnamon witches’ broom
• Identification of a phytoplasma associated
• Cinnamomum cassia B
• Phyllody phytoplasma
• Characterization of Niger
• Cymbopogon citratus
• Leaf phytoplasma
• Khasi mandarin
• Candidatus liberibacter asiaticus
• Khasi mandarin orchards
• Total DNA was extracted
• Papaya ring spot virus
• Papaya ring spot virus disease
• NCBI Genebank
• PRSV in the plants
• Virulence associated genes
• Salmonella Serovars isolated
• Raw pork of Aizawl and Imphal
• Worry from hygienic point
• China aster
• Molecular and detection
• Characterization of china aster
• 16S rII phytoplasmal group
• Latex agglutination test
• Group a rotaviruses
• Bhandara and Chandrapur district
• Circulating rotaviruses