tailieunhanh - Micropropagation of Vaccinium arctostaphylos L. via lateral-bud culture

The aim of this study was to determine the most suitable growth medium for rapid and efficient micropropagation of Vaccinium arctostaphylos L. (Ericaceae) via lateral-bud cultures. According to preliminary studies, the best explant collection time was late April and May. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 40-44 © TÜBİTAK doi: Micropropagation of Vaccinium arctostaphylos L. via lateral-bud culture Mustafa CÜCE, Ersan BEKTAŞ, Atalay SÖKMEN* Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey Received: Accepted: Published Online: Printed: Abstract: The aim of this study was to determine the most suitable growth medium for rapid and efficient micropropagation of Vaccinium arctostaphylos L. (Ericaceae) via lateral-bud cultures. According to preliminary studies, the best explant collection time was late April and May. Accordingly, cuttings were taken from naturally growing V. arctostaphylos populations, and lateral shoot buds were used as explant sources. Anderson’s rhododendron medium (AN), McCown’s woody plant medium (WPM), and Murashige and Skoog (MS) basal medium, each supplemented with zeatin/indole-3-butyric acid (IBA) ( mg L–1), were tested to determine the best basal medium for multiple shoot formation; WPM was the most effective basal medium for this purpose. Subsequent shoot multiplication and development studies were carried out with WPM supplemented with an auxin, IBA ( mg L–1), and 2 different cytokinins, zeatin and thidiazuron (TDZ), in various concentrations (, , and mg L–1, respectively). After a 6-week photoperiod (16/8 h, light/ dark) application, zeatin/IBA combinations were found to be the most suitable for shoot multiplication and growth. The highest shoot length () was obtained from the medium supplemented with mg L–1 zeatin/IBA, while the highest increases in leaf number () and multiple shoot proliferation () were obtained from medium supplemented with mg L–1 zeatin/ IBA. WPM was also employed as a rooting medium supplemented with different .