tailieunhanh - Study on the expression of dehydrin genes and activities of antioxidative enzymes in floral buds of two sand pear (Pyrus pyrifolia Nakai) cultivars requiring different chilling hours for bud break
Seasonal expression pattern of antioxidative genes (dehydrins), activities of antioxidant enzymes and H2O2 contents, and their relations to dormancy phases in floral buds of sand pear (Pyrus pyrifolia Nakai ‘Cuiguan’ and ‘Wonhwang’) were documented in the present study. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 930-939 © TÜBİTAK doi: Research Article Study on the expression of dehydrin genes and activities of antioxidative enzymes in floral buds of two sand pear (Pyrus pyrifolia Nakai) cultivars requiring different chilling hours for bud break 1 1 1 1,2 3 1, Sayed HUSSAIN , Guoqin LIU , Dongfen LIU , Maqsood AHMED , Nazim HUSSAIN ,Yuanwen TENG * Department of Horticulture, State Agricultural Ministry’s Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, Zhejiang Province, . China 2 Department of Bio-Technology, Mirpur University of Science and Technology, Mirpur, Azad Jammu and Kashmir, Pakistan 3 Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang Province, . China 1 Received: Accepted/Published Online: Printed: Abstract: Seasonal expression pattern of antioxidative genes (dehydrins), activities of antioxidant enzymes and H2O2 contents, and their relations to dormancy phases in floral buds of sand pear (Pyrus pyrifolia Nakai ‘Cuiguan’ and ‘Wonhwang’) were documented in the present study. The results showed that the expression patterns of PpDHN1, PpDHN2, and PpDHN3 in the two cultivars were similar, but the expression peak time and quantity were different. In the floral buds of Cuiguan, expression of all three dehydrin genes (PpDHN1, PpDHN2, and PpDHN3) was detected during endodormancy, reached the highest levels in ecodormancy, and then declined during the bud break stage. In the floral buds of Wonhwang, the expression of all the dehydrin genes (PpDHN1, PpDHN2, and PpDHN3) were at their highest in endodormancy and declined in ecodormancy. High activities of superoxide dismutase (SOD) were detected in endodormancy and decreased during ecodormancy, then increased again
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