tailieunhanh - Transcriptome characterization and large-scale identification of SSR/SNP markers in symbiotic nitrogen fixation crop faba bean (Vicia faba L.)

This study provides large-scale identification of the faba bean transcriptome resources for functional genomics studies and the development of molecular markers, which will certainly lead to crop improvement. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 459-469 © TÜBİTAK doi: Research Article Transcriptome characterization and large-scale identification of SSR/SNP markers in symbiotic nitrogen fixation crop faba bean (Vicia faba L.) 1,2 3 1 4 1 Sundan SURESH , Tae-Sung KIM , Sebastin RAVEENDAR , Joon-Hyeong CHO , Jung Yoon YI , 1 1 1 1 1, Myung Chul LEE , Sok-Young LEE , Hyung-Jin BAEK , Gyu-Taek CHO , Jong-Wook CHUNG * 1 National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea 2 Department of Botany, Directorate of Distance Education, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu, India 3 Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Republic of Korea 4 Department of Biological and Environmental Science, Dongguk University, Seoul, Republic of Korea Received: Accepted: Published Online: Printed: Abstract: We used 454 sequencing technology for faba bean transcriptome, which yielded Mb sequence data. A total of 81,333 raw sequence reads were obtained and assembled by de novo sequence assembly. The contig distributions in three nonmutually exclusive gene ontology classifications and clusters of orthologous gene classes were assigned, which were also used to identify genes related to nitrogen fixation. Furthermore, a set of 1729 reads with simple sequence repeat (SSR) motifs were identified. Subsets of 55 SSR primer pairs were selected to validate SSR marker assay. Fifty-five primer pairs were used to amplify from one or more of the template genotypes, the single nucleotide polymorphism (SNP) types manifested high confidence differences, and 1946 SNPs, 145 insertiondeletions, and 110 variants were observed with more than one nucleotide among the detected SNPs. This study provides