tailieunhanh - Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination
The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 48-54 © TÜBİTAK doi: Research Article Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination 1, 1 1 2 Nafiz ÇELİKTAŞ *, Murat TİRYAKİOĞLU , Ersin CAN , Duygu KUTLAY , Rüştü HATİPOĞLU 1 Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, Hatay, Turkey 2 Institute of Natural and Applied Sciences, Mustafa Kemal University, Hatay, Turkey 3 Department of Field Crops, Faculty of Agriculture, Çukurova University, Adana, Turkey Received: Accepted: Published Online: 3 Printed: Abstract: The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from different districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days after the interspecific hybridization. The highest average seed setting, embryo formation, and dihaploid plant regeneration for the different hybrid combinations were determined as , , and , respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes. Key words: Imperata cylindrica L., interspecific cross, Triticum durum L., polyhaploid 1. Introduction One of the most important problems of the pasta industry is the difficulty of obtaining high-quality durum wheat. The number of produced varieties that are capable of satisfying the quality requirements of the industry is quite limited. Therefore, breeding durum wheat varieties based on sector demand is a high priority. Doubled haploid techniques, which have .
đang nạp các trang xem trước
