tailieunhanh - Epigenetic regulation of specific transcription factors in osteogenic differentiation of mesenchymal stem cells

An abundance of experiments have been performed to clarify the differentiation process of mesenchymal stem cells (MSCs). Osteogenic differentiation and in vitro conditions favoring these cell lineages have attracted the attention of many researchers. | Turkish Journal of Biology Turk J Biol (2016) 40: 1040-1049 © TÜBİTAK doi: Review Article Epigenetic regulation of specific transcription factors in osteogenic differentiation of mesenchymal stem cells 1 2 3 4, Milad ZADI HEYDARABAD , MousaVATANMAKANIAN , Mina NIKASA , Majid FARSHDOUSTI HAGH * 1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2 Department of Hematology, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Biology, East Azerbaijan Science and Research Branch, Islamic Azad University, Tabriz, Iran 4 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Received: Accepted/Published Online: Final Version: Abstract: An abundance of experiments have been performed to clarify the differentiation process of mesenchymal stem cells (MSCs). Osteogenic differentiation and in vitro conditions favoring these cell lineages have attracted the attention of many researchers. Moreover, the gene expression profile during MSC differentiation toward its main specialized cells (bone, cartilage, and adipose cells) has been mostly understood. In the last decades another layer of investigation has attempted to clarify the epigenetic mechanisms underlying MSC differentiation into its specialized cells. It has been shown that as MSCs progress through the differentiation process, more nonspecific genes undergo DNA methylation to prevent differentiation into improper cell fates. Moreover, promoters of lineage-specific genes are strongly hypomethylated in MSCs during differentiation. The main objective of this review is to address the role of major epigenetic mechanisms such as DNA methylation, histone modifications, and noncoding RNAs, especially miRNAs, in osteoblastic differentiation of MSCs and to summarize all the previous studies that have determined the epigenetic alterations of the .