tailieunhanh - Involvement of AMP-activated protein kinase in the protective effect of melatonin against renal ischemia reperfusion injury

These results demonstrate that the effects of melatonin on renal I/R are, for the most part, linked to AMPK activation, with the exception of the Akt pathway, which seems to be independent of AMPK. | Turkish Journal of Biology Turk J Biol (2016) 40: 837-844 © TÜBİTAK doi: Research Article Involvement of AMP-activated protein kinase in the protective effect of melatonin against renal ischemia reperfusion injury 1 1 2 3 Kaouther HADJ AYED TKA , Asma MAHFOUDH BOUSSAID , Kaouther KESSABI , Rym KAMMOUN , 2 3 4 1, Imed MESSAOUDI , Sonia GHOUL MAZGAR , Joan ROSELLO CATAFAU , Hassen BEN ABDENNEBI * 1 Unit of Molecular Biology and Anthropology Applied to Development and Health (UR12ES11), Faculty of Pharmacy, University of Monastir, Monastir, Tunisia 2 Laboratory of Genetics, Bio-Diversity and Valorization of Bio-Resources (LR11ES41), Institute of Biotechnology, University of Monastir, Monastir, Tunisia 3 Laboratory of Histology and Embryology, Faculty of Dental Medicine, University of Monastir, Monastir, Tunisia 4 Unit of Experimental Hepatic Ischemia-Reperfusion, Institute of Biomedical Investigations, Higher Council of Scientific Investigations, Barcelona, Spain Received: Accepted/Published Online: Final Version: Abstract: We investigated the implication of AMP-activated protein kinase (AMPK) in melatonin-induced protection in a renal ischemia/reperfusion (I/R) model. Animals were divided into four groups: rats of the sham group were not subjected to renal I/R. Rats in the I/R group received the vehicle 30 min before renal ischemia for 1 h followed by reperfusion for 6 h. Rats in the Mel+I/R group received melatonin (10 mg/kg) 30 min before the onset of I/R. Rats in the Mel+I/R+araA group received araA (AMPK inhibitor, 100 µg/kg per min for 10 min) just before melatonin administration. The results showed that melatonin treatment induced a significant improvement in renal function, morphology, and antioxidant status, as well as Akt pathway activation. It also decreased cytolysis and endoplasmic reticulum stress. Inhibition of AMPK by araA administration completely .