tailieunhanh - Purification and properties of lipoxygenase from opium poppy seedlings (Papaver somniferum L.)

Lipoxygenase (linoleate:oxygen oxidoreductase; EC ;LOX) is a key enzyme in the signaling pathway leading to the production of biologically active compounds involved in plant growth development and stress responses. | Turkish Journal of Biology Turk J Biol (2016) 40: 772-780 © TÜBİTAK doi: Research Article Purification and properties of lipoxygenase from opium poppy seedlings (Papaver somniferum L.) 1, 1 2,3 1 Ivana HOLKOVÁ *, František BILKA , Drahomíra RAUOVÁ , Lýdia BEZÁKOVÁ Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia 2 Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia 3 Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia 1 Received: Accepted/Published Online: Final Version: Abstract: Lipoxygenase (linoleate:oxygen oxidoreductase; EC ; LOX) is a key enzyme in the signaling pathway leading to the production of biologically active compounds involved in plant growth development and stress responses. In this study the novel LOX enzyme from opium poppy (Papaver somniferum L.) seedlings was purified to electrophoretic homogeneity and characterized. LOX activity during the germination of opium poppy seeds was analyzed, and the highest LOX activity was determined on the fourth day of germination. Opium poppy LOX was purified from 4-day-old poppy seedlings after ammonium sulfate precipitation followed by hydrophobic chromatography (Phenyl-Sepharose CL-4B), ion exchange chromatography (Q-Sepharose), and affinity chromatography (linoleyl-aminopropyl agarose) for the first time. The relative molecular weight of purified LOX was estimated to be 78 kDa by immunoblotting. The highest enzyme activity occurred at pH and mM calcium ion concentration. LOX showed preferential activity towards linoleic acid followed by linolenic acid as a substrate. To investigate the positional specificity of the LOX reaction, purified LOX was incubated with .

• Sinh học
• Enzyme purification
• Biochemical characterization
• Positional specificity
• Papaver somniferum
• Biologically active
• Purification of phytase enzyme
• Phytase enzyme
• Four selected Indian wheat varieties
• Ammonium sulphate precipitation
• Ion exchange chromatography
• Enzyme activity
• Optimal pH
• Partial purification
• Intestinal triglyceride lipase
• Polyethylene glycol
• Labeo rohita
• Acidic proteases
• Alkaline proteases
• Enzyme purification fold
• Enzyme kinetics
• BMC Biotechnology
• Recombinant protein
• Amebic cysteine protease
• Expression and purification
• Cytosolic oxidative folding
• Proteolytic activity
• Enzyme inhibition
• Biocontrol agent
• Submerged fermentation
• Cultural conditions of submerged fermentation
• Alkaline chitinase enzyme
• Fusarium sp
• scientific reports
• Report medicine
• traditional medicine
• Crystal structure
• medical analysis
• biochemical studies
• Journal of Chemistry
• Lipolytic enzymes
• Aureobasidium pullulans
• Intracellular fructosyltransferase
• Original activity
• Enzyme preparations
• Thioredoxin reductase
• Characterization purification
• Rainbow trout
• Metal ions
• Cellobiose dehydrogenase
• Sclerotium rolfsii
• Lactobionic acid
• Kiwifruit enzyme
• Cottage cheese
• Total bacterial count
• Cottage cheese produced
• Carbonic anhydrase I
• Carbonic anhydrase II
• Rosmarinic acid
• Affinity chromatography
• Chalcalburnus tarichi
• Meleagris gallopavo
• Thioredoxin protein (Trx)
• Lineweaver–Burk plots
• Pseudomonas fluorescens
• Extracellular lipase
• P nitrophenyl esters
• Bacterial enzymes
• Alkaline protease
• Bacillus cereus AG1
• Detergent compatibility
• Soil isolate bacillus cereus AG1
• Maximum production of enzyme
• Alkaliphilic Bacillus
• 16S rRNA gene sequence
• phylogenetic analysis
• Non chromatographic purification
• Catalytic efficiency
• Elastin like polypeptides
• Enzyme engineering
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• Dual gene expression
• Purification of recombinant enzyme
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• Peniophora sp
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