tailieunhanh - Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. | Turkish Journal of Biology Turk J Biol (2016) 40: 410-419 © TÜBİTAK doi: Research Article Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells 1,2, 1,2 3 1,4 1,2,5 Sevil KÖSE *, Fatima AERTS KAYA , Emir Baki DENKBAŞ , Petek KORKUSUZ , Fahriye Duygu ÇETİNKAYA 1 Department of Stem Cell Sciences, Institute of Health Sciences, Hacettepe University, Ankara, Turkey 2 Center for Stem Cell Research and Development-PEDISTEM, Hacettepe University, Ankara, Turkey 3 Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey 4 Department of Histology and Embryology, Hacettepe University, Ankara, Turkey 5 Pediatric Hematology, BMT Unit, Hacettepe University Children’s Hospital, Ankara, Turkey Received: Accepted/Published Online: Final Version: Abstract: Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. PHB membranes of random (r-PHB) or aligned (a-PHB) electrospun nanofibers that generate bone and spinal axon scaffolds were combined with human MSCs. The adhesion and proliferation of cells on these membranes were examined. The orientation of cells on PHB membranes was analyzed by confocal microscopy and scanning electron microscopy (SEM). The MSCs maintained their characteristic properties on the membranes, adhered to the membranes, and preserved their viability. The cell morphology was different when they were grown on differentially designed matrices. Cells expanded on the a-PHB membrane and showed fibroid-like morphology. Conversely, cells interacting with the r-PHB membrane were located homogeneously and demonstrated polygonal morphology. The adhesion and proliferation of human MSCs was higher on the a-PHB membrane than on