tailieunhanh - Salinity effects on expression of some important genes in sensitive and tolerant grape genotypes
The effects of NaCl on expression of some genes related to salinity tolerance in grape (Vitis L.) were investigated. According to our screening study on eighteen grape genotypes, H6 and Gharashani (tolerant) and Shirazi and GhezelUzum (sensitive) were selected for molecular analysis. | Turkish Journal of Biology Turk J Biol (2016) 40: 95-108 © TÜBİTAK doi: Research Article Salinity effects on expression of some important genes in sensitive and tolerant grape genotypes 1, 2 2 2,3 Nayer MOHAMMADKHANI *, Reza HEIDARI , Nasser ABBASPOUR , Fatemeh RAHMANI 1 Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran 2 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran 3 Biotechnology Research Center, Urmia University, Urmia, Iran Received: Accepted/Published Online: Final Version: Abstract: The effects of NaCl on expression of some genes related to salinity tolerance in grape (Vitis L.) were investigated. According to our screening study on eighteen grape genotypes, H6 and Gharashani (tolerant) and Shirazi and GhezelUzum (sensitive) were selected for molecular analysis. Our tolerant genotypes showed a higher water potential and a lower MDA content compared to other genotypes. Plants were treated with 50 mM NaCl as a critical concentration that is not lethal for grape. The expression profile of VvNHL1 in leaves of all genotypes and in roots of tolerant genotypes was similar to that of VvEDS1; these genes probably associated in defense responses. VvP450 and VvCPN21 transcripts were expected only in leaves, but they were also detected in roots. VvChS and VvPAL transcripts accumulated significantly (P 50 mM NaCl) for more than several days. Leaf and root tissues were collected at different time points (0, control; 24 h, short-time salinity; 14 days, longtime, regarding tolerance of our sensitive genotypes), immediately frozen in liquid nitrogen, and stored at –80 °C until RNA isolation. . Determination of water potential and MDA content Leaf water potential was measured by pressure chamber (Scholander et al., 1965) at different time points. Malondialdehyde (MDA) was determined by TBA reaction, as described by .
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