tailieunhanh - Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles

In this paper we present the construction of a highly sensitive FCS instrument and the measurement results from a sample of semiconductor quantum dots. We provide the analysis procedure for determining the hydrodynamic radius of the quantum dots and compare the results with the ones obtained directly from electron microscope imaging. The good agreement indicates the reliability of the FCS technique and open the way for further applications of this technique in studying nanoparticles. | Communications in Physics, Vol. 25, No. 1 (2015), pp. 59-65 DOI: DEVELOPMENT OF A FLUORESCENCE CORRELATION SPECTROSCOPY INSTRUMENT AND ITS APPLICATION IN SIZING QUANTUM DOT NANOPARTICLES NGUYEN THI THANH BAO AND DINH VAN TRUNG Institute of Physics, Vietnam Academy of Science and Technology 10 DaoTan, BaDinh, Hanoi, Vietnam E-mail: ntbao@ Received 08 December 2014 Accepted for publication 06 March 2015 Abstract. Fluorescence correlation spectroscopy is a relatively new technique to measure and quantify the statistical fluctuations of the fluorescence signal from the measurement volume. Combining with sensitive detection method and confocal microscopy, the FCS technique has become a powerful tool in studying the dynamic properties of nanoparticles at single particle level. In this paper we present the construction of a highly sensitive FCS instrument and the measurement results from a sample of semiconductor quantum dots. We provide the analysis procedure for determining the hydrodynamic radius of the quantum dots and compare the results with the ones obtained directly from electron microscope imaging. The good agreement indicates the reliability of the FCS technique and open the way for further applications of this technique in studying nanoparticles. Keywords: FCS; quantum dots; hydrodynamic radius; photon counting I. INTRODUCTION Characterization of nanoparticles has recently found wide applications in several important research fields such as nanotechnology, biophysics, chemistry etc. . . In many instances, the nanoparticles being studied exist in liquid medium such as solution or intra-cellular environment. Direct measurement of freely diffusing nanoparticles in these cases provides a convenient and noninvasive way to obtain data in real-time, where other characterization methods such as direct imaging are unsuitable. In this aspect, the fluorescence correlation spectroscopy (FCS) technique has become the method of .

• Vật lý
• Communications in Physics
• Development of a fluorescence correlation spectroscopy instrument
• Application in sizing quantum dot nanoparticles
• Sizing quantum dot nanoparticles
• The dynamic properties of nanoparticle
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