tailieunhanh - Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium

The androgenic potentials of various cucumbers were significantly (P < ) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. | Turkish Journal of Biology Turk J Biol (2016) 40: 571-579 © TÜBİTAK doi: Research Article Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium 1, 1 1 2 1 Mohammad Reza ABDOLLAHI *, Samereh NAJAFI , Hasan SARIKHANI , Sayyed Saeed MOOSAVI Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran 2 Department of Horticultural sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran Received: Accepted/Published Online: Final Version: Abstract: Anther culture response of a cucumber cultivar (Beta Alpha) and three Iranian landraces of cucumber (Basmenj, Isfahani, and Korki) on MS culture media with an altered macronutrient strength (half, full, , and double-strength) or various concentrations of agar (0, , 7, and 14 g L–1) was evaluated in two separate experiments. The effect of different concentrations of agar on the development of callus-derived embryos was also assessed. The androgenic potentials of various cucumbers were significantly (P < ) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. A doubled strength of macronutrients in culture medium resulted in the highest percentage of callogenesis (100%) in Isfahani cucumber, while use of full strength of macronutrients produced the highest percentage of embryogenic calli () in the mentioned landrace compared to other tested forms. The maximum number of gametic embryos ( and embryos/anther) differentiated from Isfahani anther cultures on full- and half-strength MS medium, respectively. In the second experiment, the highest percentages of callogenesis were obtained in MS culture media solidified with 14 g L–1 and 7