tailieunhanh - Expression profiles of genes related to melatonin and oxidative stress in human renal proximal tubule cells treated with antibiotic amphotericin B and its modified forms

Melatonin protects cells from oxidative stress caused by antibiotics. Through the generation of reactive oxygen species (ROS), amphotericin B (AmB) causes oxidative injuries of the kidneys and liver. Generation of oxidized forms of AmB (AmB-ox) in a patient’s circulation may also generate ROS, causing side effects. | Turkish Journal of Biology Turk J Biol (2015) 39: 856-864 © TÜBİTAK doi: Research Article Expression profiles of genes related to melatonin and oxidative stress in human renal proximal tubule cells treated with antibiotic amphotericin B and its modified forms 1, 1 1 1 1 Joanna GOLA *, Aleksandra SKUBIS , Bartosz SIKORA , Celina KRUSZNIEWSKA-RAJS , Jolanta ADAMSKA , 1 1 2 3 Urszula MAZUREK , Barbara STRZAŁKA-MROZIK , Grzegorz CZERNEL , Mariusz GAGOŚ 1 Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland 2 Department of Biophysics, University of Life Sciences in Lublin, Poland 3 Department of Cell Biology, Institute of Biology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland Received: Accepted/Published Online: Printed: Abstract: Melatonin protects cells from oxidative stress caused by antibiotics. Through the generation of reactive oxygen species (ROS), amphotericin B (AmB) causes oxidative injuries of the kidneys and liver. Generation of oxidized forms of AmB (AmB-ox) in a patient’s circulation may also generate ROS, causing side effects. Studies aimed at elimination of toxic properties of AmB are focused on forming complexes of AmB with copper(II) ions (AmB–Cu2+). However, the influence of such modified forms on renal cells and their transcriptome has not been studied so far. Therefore, the aim of this study was to answer the question of whether AmB–Cu2+ complexes and AmB-ox influence the transcriptional activity of melatonin-related and oxidative stress-related genes in human renal proximal tubule epithelial cells (RPTECs) and whether these changes can result in less toxicity of modified forms of AmB. Gene expression profile was evaluated with the use of oligonucleotide microarrays. At high concentrations AmB–Cu2+ was two times less toxic .