tailieunhanh - Genetic diversity and molecular characterization of natural Pancratium maritimum L. populations by DNA markers

Bulbous plants play an important role in Turkey’s biodiversity due to their great potential use in various industries. Sea daffodils (Pancratium maritimum), one aspect of Turkey’s biological richness, represent an important bulbous plant that generally spreads on sand dunes and can be seen only on the Mediterranean coast and on its certain beaches in our country. | Turkish Journal of Botany Turk J Bot (2017) 41: 569-578 © TÜBİTAK doi: Research Article Genetic diversity and molecular characterization of natural Pancratium maritimum L. populations by DNA markers 1 2, Ceren ELİBOL , Behiye Banu BİLGEN * Department of Agricultural Biotechnology, Faculty of Agriculture, Eskişehir Osmangazi University, Eskişehir, Turkey 2 Department of Agricultural Biotechnology, Faculty of Agriculture, Namık Kemal University, Tekirdağ, TURKEY 1 Received: Accepted/Published Online: Final Version: Abstract: Bulbous plants play an important role in Turkey’s biodiversity due to their great potential use in various industries. Sea daffodils (Pancratium maritimum), one aspect of Turkey’s biological richness, represent an important bulbous plant that generally spreads on sand dunes and can be seen only on the Mediterranean coast and on its certain beaches in our country. In this study, the genetic structure and genetic diversity of four natural P. maritimum populations have been determined by RAPD and nrSSR primers. Eight RAPD and four nrSSR loci were analyzed. All RAPD and nrSSR loci, except SSR-20, were found to be polymorphic. Genetic diversity parameters such as mean number of alleles for each nrSSR loci (Na = ), effective allele number (Ne = ), Shannon’s information index (I = ), observed heterozygosity (Ho = ) and expected heterozygosity (He = ) were calculated. A rather high proportion of the genetic diversity (81% for nrSSR, 72% for RAPD) was due to within-population variation and the remaining part was due to variation between populations. According to the acquired UPGMA phenogram for RAPD and nrSSR data, the İğneada and Çamlıkoy populations, which are geographically close, are also genetically the most similar populations. The STRUCTURE analysis results supported the constructed UPGMA phenogram for the studied sea .

• Nông nghiệp
• Genetic conservation
• Genetic diversity
• Pancratium maritimum
• Sea daffodil
• Bulbous plant
• Evolutionary Conservation
• conservation biology
• Conservation units
• Phylogenetics
• genetic variation
• neutral variation
• Nucleotide diversity
• Threatened cycads (cycadaceae)
• Conservation of genetic diversity
• High propagules
• Levels of genetic diversity
• Plant Biology
• Conservation strategy
• Genetic relationships
• Paeonia decomposita
• Population structure
• Simple sequence repeat
• Genetic variability
• Phylogenetic relationship
• Little millets Panicum sumatrense
• Genetic variability among
• Genetic variation in threatened conifer
• Cunninghamia lanceolata var
• Konishii using ISSR markers
• Implications for conservation
• Species conservation
• BMC Genetics
• Loropetalum subcordatum
• Genetic structure
• Ex situ population
• Plant conservation
• Reproductive Science
• Integrated Conservation
• genetic management
• embryo technologies
• predator control
• reproductive technologies
• Evolutionary genetics
• natural populations
• Factors controlling
• environment interaction
• Genetic consequences
• genetic drift
• Breonadia salicina
• Molecular markers
• Genetic variation and outcrossing rate
• Dipterocarpus Dyeri
• The Tan Phu tropical rain forest
• Appemidi mango
• Conservation and genotypes
• Appemidi mango (Mangifera indica L
• Genetic diversity of appemidi mango
• Significance of bioinformatics
• Conservation of biodiversity
• Biodiversity and databases
• Correlate genetic information
• Genealogical relationships
• Conservation genetics
• Landscape permeability
• Functional habitat connectivity
• Dendrometric diversity
• Genetic diversity of this tree species
• Principal component analysis
• Quercus suber L
• Plant genetic resources
• Exotic germplasm
• Forage yield
• Human consumption
• Grain yield
• Genome Biology
• Conservation genomics
• Lynx pardinus
• Ex situ conservation
• Genetic diferentiation
• Single nucleotide polymorphisms
• Breeding materials
• Analysis of molecular variance
• Marfan syndrome
• Genetic testing
• Fibrillin 1 gene
• Disease stratification
• Grantham score
• Conservation across species
• Roundscale spearfish
• White marlin
• Genetic population structure
• Effective population size
• Tetrapturus georgii
• Kajikia albida
• Bilaterally symmetrical fruits
• Acer platanoides L
• Morphogenetic control
• Norway maple
• Climate change
• Gene conservation
• Agricultural species
• Minimizing fragmentation
• Increase genetic
• Heritability studies
• Single tuberose genotypes
• Conservation of germplasm
• Achieve characterization
• Journal of Botany
• Ferula sinkiangensis
• Genetic assessment
• Small population size
• Medicinal plant
• Plant’s natural habitats
• BMC Plant Biology
• Cycas simplicipinna
• Genetic differentiation
• Population contraction
• In situ