tailieunhanh - Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts

Moringa oleifera Lam. and Centella asiatica (L.) Urb. leaves have been previously reported to exhibit antioxidant activity. The objective of the present study is to determine the in vitro antioxidant activity of the combined extracts of M. oleifera and C. asiatica (TGT-PRIMAAGE) and its effect on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts. | Turkish Journal of Biology Turk J Biol (2018) 42: 33-44 © TÜBİTAK doi: Research Article Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts 1,2 1 1 1 Elly Ezlinda ABDUL HISAM , Mohd Salleh ROFIEE , Aina Mardhia KHALID , Ahmad Firdaus JALALUDDIN , 1,2 1,2 1,2 1,2 1 Mohd Izwan MOHAMAD YUSOF , Muhd Hanis IDRIS , Salfarina RAMLI , Richard Johari JAMES , Wee JACK YOENG , 1,2 1,2, Teh LAY KEK , Mohd Zaki SALLEH * 1 Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor, Puncak Alam, Malaysia 2 Faculty of Pharmacy, Universiti Teknologi MARA Selangor, Puncak Alam, Malaysia Received: Accepted/Published Online: Final Version: Abstract: Moringa oleifera Lam. and Centella asiatica (L.) Urb. leaves have been previously reported to exhibit antioxidant activity. The objective of the present study is to determine the in vitro antioxidant activity of the combined extracts of M. oleifera and C. asiatica (TGT-PRIMAAGE) and its effect on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts. TGTPRIMAAGE acted on the mechanism of hydrogen transfer as it showed scavenging activity in the DPPH assay. This is due to the presence of phenolics and flavonoids in TGT-PRIMAAGE. TGT-PRIMAAGE effectively reduced cellular generation of reactive oxygen species induced by H2O2. The activities of superoxide dismutase and catalase were also increased in cells treated with TGT-PRIMAAGE. Treatment with TGT-PRIMAAGE showed significant reduction (P ) effects of TGT-PRIMAAGE at various concentrations until 72 h of incubation. Therefore, three different concentrations were chosen to investigate the inhibitory effect of TGTPRIMAAGE on H2O2-induced senescence in HDFs, which were 125, 250, and 500 µg/mL. . Effect of TGT-PRIMAAGE on ROS, SOD, and