tailieunhanh - Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells

Galectins (gals) are β-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. | Turkish Journal of Biology Turk J Biol (2017) 41: 843-848 © TÜBİTAK doi: Research Article Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells* 1, 1 2, , 1, 1 2 Danica ĆUJIĆ *, Maja KOSANOVIĆ * **, Milica JOVANOVIĆ KRIVOKUĆA *, Ljiljana VIĆOVAC , Miroslava JANKOVIĆ Department of Biology of Reproduction, Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Belgrade, Serbia 2 Department of Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Belgrade, Serbia Received: Accepted/Published Online: Final Version: Abstract: Galectins (gals) are β-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin mRNA content was determined by real-time PCR. The presence of galectins in fractions of HTR8/SVneo CM and EVs was detected by western blot. All three galectins expressed by HTR-8/SVneo cells (gal-1, gal-3, and gal-8) were detected in the CM, HMF, and EVs, while only gal-1 was found in the LMF. In addition, EVs contained all three galectin mRNAs. These results reveal that free, complexed, and EV-associated forms of galectins were released from extravillous trophoblast cells, suggesting their potential to exert extracellular functions both in their .

• Sinh học
• β galactoside
• Trophoblast cell line galectins
• Extracellular vesicles
• Conditioned medium
• Molecular mass fraction
• Scientific reports
• scientific research
• molecular Biology
• medicine
• cell proliferation
• oxidation