tailieunhanh - N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering

In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. | Turkish Journal of Biology Turk J Biol (2017) 41: 796-807 © TÜBİTAK doi: Research Article N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering 1, 2 3 Murat DEMİRBİLEK *, Nelisa TÜRKOĞLU LAÇİN , Selçuk AKTÜRK Advanced Technologies Research and Application Center, Hacettepe University, Ankara, Turkey 2 Science and Technology Application and Research Center, Yıldız Technical University, İstanbul, Turkey 3 Department of Physics, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels. Additionally, increasing the NAG concentration decreased elastic modulus and degradation time. Hydrogels were not cytotoxic on HaCaT and HUVEC cells. It stimulated IL-10 and TNF-alpha levels at a small rate. Key words: Biomaterials, alginate hydrogels, hyaluronic acid monomers, cytokines 1. Introduction After trauma or surgery, the disruption of the normal anatomical and functional integrity of the skin is called a wound. Some postinjury events are intended to restore tissue integrity regarding functional and .