tailieunhanh - Age- and sex-dependent alteration of functions and epigenetic modifications of vessel and endothelium related biomarkers

Aging is a main risk factor for development of cardiovascular diseases associated with the impairment of endothelial function in both sexes. In the present study, age-related changes in vascular responsiveness, epigenetic modifications of vessel wall, and blood biomarkers related to endothelial functions were examined in an age- and sex-dependent manner. | Turkish Journal of Biology Turk J Biol (2018) 42: 286-296 © TÜBİTAK doi: Research Article Age- and sex-dependent alteration of functions and epigenetic modifications of vessel and endothelium related biomarkers 1,* 2 3 3 Sevtap HAN , Muammer Merve AYDIN , Serdar AKANSEL , Suzan Emel USANMAZ , 2 1 3 Can AKÇALI , Mecit Orhan ULUDAĞ , Emine DEMİREL YILMAZ 1 Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey 2 Department of Biophysics, Faculty of Medicine, Ankara University, Ankara, Turkey 3 Department of Medical Pharmacology, Faculty of Medicine, Ankara University, Turkey Received: Accepted/Published Online: Final Version: Abstract: Aging is a main risk factor for development of cardiovascular diseases associated with the impairment of endothelial function in both sexes. In the present study, age-related changes in vascular responsiveness, epigenetic modifications of vessel wall, and blood biomarkers related to endothelial functions were examined in an age- and sex-dependent manner. Acetylcholine (ACh)-induced relaxations of the aorta were decreased in 3-, 6-, and 12-month-old rats compared to those in 1-month-old female rats. In males, maximum relaxations related to ACh were higher in 1- and 6-month-old rats than in 3- and 12-month-old rats. Plasma levels of nitric oxide (NO) and asymmetric dimethylarginine (ADMA) decreased with age in female rats, and total antioxidant capacity (TAC) and hydrogen sulfide (H2S) levels displayed biphasic alterations. In male rats, plasma levels of NO, TAC, and ADMA decreased with age, and H2S levels increased. Aging also caused a sex-dependent alteration in epigenetic modification of vessels. Expressions of H3K27me2, H3K27me3, H3K36me2, and H3K36me3 were much higher in vessels of 12-month-old female rats compared to those in younger age groups. These results indicate that vascular functions, .