tailieunhanh - Chemical constituents of the ethyl acetate fraction of the fruit bodies of phellinus gilvus

Five fungal secondary metabolites named 1,2,4,5-tetrachloro-3,6-dimethoxybenzene (1); ergosterol (2); ergosterol peroxide (3); (E)- 4-(3,4-dihydroxyphenyl)but-3-en-2-one (4) and [bi1,4-cyclohexandien-1-yl]-3,3’,6,6’-tetrone, 4, 4’-dihydroxyl-2,2’,5,5’-tetramethyl (5) were purified from the ethyl acetate fraction extract of the methanol extract fruit bodies of Phellinus gilvus (Schwein.) Pat. | Vietnam Journal of Science and Technology 56 (4A) (2018) 246-251 CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE FRACTION OF THE FRUIT BODIES OF PHELLINUS GILVUS Do Xuan Hung1, Luu Hang Nga2, Tran Dinh Thang3, Dang Ngoc Quang2, * 1 School of Natural Sciences Education, Vinh University, 182 Le Duan, Vinh city, Nghe An province 2 Faculty of Chemistry, Hanoi National University ofEducation, 136 Xuan Thuy, Cau Giay district, Ha Noi 3 School of Chemistry, Biology and Enviroment, Vinh University, 182 Le Duan, Vinh City, Nghe An province * Email: quangdn@ Received: 13 July 2018; Accepted for publication: 30 October 2018 ABSTRACT Five fungal secondary metabolites named 1,2,4,5-tetrachloro-3,6-dimethoxybenzene (1); ergosterol (2); ergosterol peroxide (3); (E)- 4-(3,4-dihydroxyphenyl)but-3-en-2-one (4) and [bi1,4-cyclohexandien-1-yl]-3,3’,6,6’-tetrone, 4, 4’-dihydroxyl-2,2’,5,5’-tetramethyl (5) were purified from the ethyl acetate fraction extract of the methanol extract fruit bodies of Phellinus gilvus (Schwein.) Pat. collected at Pu Mat national park, Nghe An province. Their structures were characterized by 1D, 2D NMR and GC-MS spectroscopies. Especially, the structure of compound 1 was confirmed by X-ray crystallographic analysis. This is the first report on the chemical constituents of Vietnamese Phellinus gilvus. Keywords: Phellinus gilvus, metabolites, antimicrobial, cytotoxicity. 1. INTRODUCTION It is estimated that there are totally million mushrooms species globally, about 22000 species are found in Vietnam. They play a very important role not only for ecosystem but also for our human life. From ancient time, many mushrooms have been used in traditional medicines to treat various diseaes [1]. The mushroom Phellinus gilvus (Schwein.) Pat. widely grows in Asia, North America and Africa. Its major chemical constituents are anticancer polysaccharides [2, 3], together with other secondary metabolies such as antioxidant phenollic compounds .