tailieunhanh - Storage behavior and cryopreservation studies in Indian rough lemon (Citrus jambhiri): a promising rootstock for long-term conservation

The study of the seed storage behavior and cryopreservation of embryonic axes was attempted in Citrus jambhiri genotypes using air desiccation-freezing, vitrification, and encapsulation-dehydration methods. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2016) 40: 865-873 © TÜBİTAK doi: Research Article Storage behavior and cryopreservation studies in Indian rough lemon (Citrus jambhiri): a promising rootstock for long-term conservation 1 2 1, 2 1 2 Mony Radhika ROHINI , Surendra Kumar MALIK , Ravish CHOUDHARY *, Sukhdeep KAUR , Ajit UCHOI , Rekha CHAUDHURY 1 Indian Agricultural Research Institute, Pusa Campus, New Delhi, India 2 Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India Received: Accepted/Published Online: Final Version: Abstract: The study of the seed storage behavior and cryopreservation of embryonic axes was attempted in Citrus jambhiri genotypes using air desiccation-freezing, vitrification, and encapsulation-dehydration methods. Seeds of this species revealed nonorthodox seed storage behavior with moderate seed longevity and sensitivity to desiccation and freezing. Successful cryopreservation was achieved with all three methods using embryonic axes; however, the recovery growth percentage significantly differed among these methods. Cryopreservation was efficient for the embryonic axes desiccated to 13%–15% moisture content. Freezing of embryonic axes at this moisture content in liquid nitrogen showed a good recovery rate of 40%–50%. In the vitrification method, embryonic axes subjected to PVS2 treatment for 40 min had the highest recovery rate of 25% after cryopreservation. In the encapsulation-dehydration method, encapsulated axes precultured on M sucrose medium followed by 6 h of desiccation had the highest recovery growth of 30%– 60% after cryopreservation. Out of the three methods employed, the encapsulation-dehydration method gave the best recovery values for embryonic axes. High regeneration values of embryos in comparison to embryonic axes prompted us to .