tailieunhanh - First report of “Candidatus Phytoplasma solani” on a new host marigold (Tagetes erecta L.)

Marigold (Tagetes erecta L.) plants, also called Mexican or Aztec marigold, with symptoms of shoot proliferation, dwarfing and reddening were observed in ornamental gardens of Van Province (Turkey). | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2016) 40: 311-318 © TÜBİTAK doi: First report of “Candidatus Phytoplasma solani” on a new host marigold (Tagetes erecta L.) 1 2 3, 2 Şevket ALP , Mustafa USTA , Hikmet Murat SİPAHİOĞLU *, Abdullah GÜLLER Department of Landscape Architecture, Faculty of Agriculture, Yüzüncü Yıl University, Van, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Yüzüncü Yıl University, Van, Turkey 3 Department of Plant Protection, Faculty of Agriculture, Inönü University, Battalgazi, Malatya, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: Marigold (Tagetes erecta L.) plants, also called Mexican or Aztec marigold, with symptoms of shoot proliferation, dwarfing, and reddening were observed in ornamental gardens of Van Province (Turkey). Five plants, two of them showing reddening and three symptomless plants, were sampled at the end of September 2014. Genomic DNA isolated from symptomatic and nonsymptomatic plant leaves was used to amplify 16S rDNA fragments by nested polymerase chain reaction (PCR). Of the 5 marigold samples tested by PCR, only the two showing reddening symptoms yielded the expected DNA fragments. Amplified PCR fragments were cloned into a plasmid vector and transformed into competent Escherichia coli strain JM 109. Recombinant plasmid DNA was isolated and sequenced bidirectionally. The provided sequences were 1244 bp and 1245 bp in length and were designated as isolate 1 and isolate 2, respectively. BLAST analysis of the 16S rDNA sequence and virtual restriction fragment length polymorphism (RFLP) analysis confirmed the presence of the phytoplasma “Candidatus Phytoplasma solani”. The in silico virtual RFLP pattern of isolate 1, based on the 16S rDNA F2n/R2 fragment, was identical (similarity coefficient ) to the reference pattern of 16Sr