tailieunhanh - A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco

The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). | Turkish Journal of Biology Turk J Biol (2017) 41: 608-621 © TÜBİTAK doi: Research Article A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco 1,2, 1 2 Tian LI *, Jingkuan SUN , Yuping BI Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Shandong, . China 2 Biotechnology Research Center, Shandong Academy of Agricultural Science; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Shandong, . China 1 Received: Accepted/Published Online: Final Version: Abstract: The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of stress- and ABA-related genes after NaCl treatment. These results indicated that FvMYB2 may play a positive role in salt stress regulation mediated by ABA-dependent signaling mechanisms. Key words: Ash, MYB transcription factor, transcription, salt stress 1. Introduction High salinity usually causes osmotic stress and ion injury in plant cells, which seriously affects the growth and development of crops and trees (Barnabas et al., 2008). After a long period of biological evolution, plants have developed a variety of mechanisms to adapt to this environmental stress .