tailieunhanh - High dietary fructose load aggravates lipid metabolism in the liver of Wistar rats through imbalance between lipogenesis and fatty acid oxidation

Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β-oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. | Turkish Journal of Biology Research Article Turk J Biol (2016) 40: 1235-1242 © TÜBİTAK doi: High dietary fructose load aggravates lipid metabolism in the liver of Wistar rats through imbalance between lipogenesis and fatty acid oxidation Ana TEOFILOVIĆ, Biljana BURSAĆ, Ana DJORDJEVIC, Danijela VOJNOVIĆ MILUTINOVIĆ, Gordana MATIĆ, Nataša VELIČKOVIĆ* Department of Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia Received: Accepted/Published Online: Final Version: Abstract: Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β‑oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-weeklong consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The expression of key regulators of fatty acid oxidation (FAO) and lipogenesis in the liver were assessed by western blot and quantitative polymerase chain reaction. The results showed that fructose-fed rats were normoglycemic and hypertriglyceridemic with visceral adiposity, but without hepatic lipid deposition. A high-fructose diet is associated with increased nuclear levels of the lipogenic regulator sterol regulatory element binding protein 1c (SREBP-1c), which was followed by increased acetyl‑CoA carboxylase and fatty acid synthase mRNAs. The nuclear level of the FAO transcriptional regulators peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1) and lipin‑1 were unaltered, while carnitine palmitoyltransferase 1 (CPT1) mRNA was significantly decreased. Overall, our findings showed that liquid fructose overconsumption is

• Sinh học
• Dietary fructose
• Fatty acid oxidation
• Hepatic steatosis
• Fatty acid β oxidation
• Fatty acid oxidation
• Scientific reports
• breast cancer
• medicine
• oxidation
• disorders
• Fatty acid
• BMC Pediatrics
• Fatty acid oxidation disorders
• Newborn screening
• Genotype phenotype correlation
• Treatment outcome
• BMC Cancer
• Gene signature
• Tumour normal
• Oestrogen receptor
• Rhododendron oldhamii
• Free fatty acid
• Fat accumulation
• High fat diet
• Non alcoholic fatty liver disease
• Principles of biochemistry
• Lecture Principles of biochemistry
• Nguyên lý hóa sinh
• Biochemical compounds
• Fatty acid catabolism
• Gene transcription
• Steroid hormone receptor
• Tumor marker
• Report medicine
• traditional medicine
• Crystal structure
• bacteria
• thesis report
• medical report
• Structural biochemistry
• acid activation
• Energy yield
• Oxidation of palmitate
• Odd chain
• fatty acids
• Palmitate synthesis
• biological activities
• gastric cancer
• biochemical studies
• environmental medicine
• Organic acidemia
• Tandem mass spectrometry
• Inborn errors of metabolism
• Carnitine palmitoyl transferase 1A
• Clinical significance
• Healthy controls
• BMC Genomics
• Fat body
• Ribosomal profiling
• Fatty acid beta oxidation
• Oxidative stress response
• cell proliferation
• Beta thalassemia major
• Impaired acylcarnitine profile
• Transfusion dependent
• Beta thalassemia major patients
• North China
• Multi ethnic region
• Several generations
• Colorectal cancer
• T cell immunoglobulin and mucin domain 3
• Gene set variant analysis
• Fatty acid alpha oxidation
• L asparaginase
• Mitochondrial respiration
• Fatty acid oxidation
• Mitochondrial membrane potential