tailieunhanh - Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment

This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves. Following foliar spray at three growth stages (40, 50, and 60 days) with selected PGRs, namely humic acid (HA), Moringa oleifera leaf extract (MOLE), and 6-benzylamino purine (6-BAP), and their mixed form, the samples of spinach leaves were harvested. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2016) 40: 136-145 © TÜBİTAK doi: Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment 1,2 1, 3,4 5 Maryam ASLAM , Bushra SULTANA *, Farooq ANWAR , Hassan MUNIR Department of Chemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan 2 Department of Chemistry, Government College Women University, Faisalabad, Pakistan 3 Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Kharj, Saudi Arabia 4 Department of Chemistry, University of Sargodha, Sargodha, Pakistan 5 Department of Crop Physiology, University of Agriculture Faisalabad, Faisalabad, Pakistan 1 Received: Accepted/Published Online: Final Version: Abstract: This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves. Following foliar spray at three growth stages (40, 50, and 60 days) with selected PGRs, namely humic acid (HA), Moringa oleifera leaf extract (MOLE), and 6-benzylamino purine (6-BAP), and their mixed form, the samples of spinach leaves were harvested. The analysis of foliar-treated leaves revealed the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be – µg/g FW, – ng/g FW, – µg/g FW, – mg/g FW, and – mg/g FW, respectively. The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from to mg GAE/g DM, from to ( mg/ mL extract concentration), and from to µg/mL, respectively. HPLC analysis showed that foliar application of PGRs also improved the content of .