tailieunhanh - Evaluation of disease intensity and molecular identification of Zymoseptoria tritici causing Septoria leaf blotch on wheat in the Eastern Mediterranean Region of Turkey

Wheat is one of the most important crops in Turkey. Production of this crop is constrained by the disease Septoria leaf blotch, which is a major bottleneck in wheat production in the Eastern Mediterranean Region of Turkey. In this study, 260 different wheat fields were surveyed for the estimation of prevalence and severity of Septoria leaf blotch. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2017) 41: 405-413 © TÜBİTAK doi: Evaluation of disease intensity and molecular identification of Zymoseptoria tritici causing Septoria leaf blotch on wheat in the Eastern Mediterranean Region of Turkey 1 2, 1 2 Gülsüm ÜNAL , Mukaddes KAYIM *, Tahsin AY , Amin Mohammed YONES 1 Biological Control Research Institute, Yüreğir, Adana, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Çukurova University, Sarıçam, Adana, Turkey Received: Accepted/Published Online: Final Version: Abstract: Wheat is one of the most important crops in Turkey. Production of this crop is constrained by the disease Septoria leaf blotch, which is a major bottleneck in wheat production in the Eastern Mediterranean Region of Turkey. In this study, 260 different wheat fields were surveyed for the estimation of prevalence and severity of Septoria leaf blotch. Out of 100% of the total wheat crop fields, were infected with this disease. The mean value of Septoria leaf blotch virulence was . Out of 142 diseased wheat crop samples, 74 isolates were used for the pathogenicity test. Forty-seven isolates from Adana, 3 isolates from Mersin, 3 isolates from Osmaniye, 14 isolates from Hatay, and 7 isolates from Kahramanmaraş provinces were cultured on PDA plates. The cause of disease agent was identified based on microscopic observation of morphological structure and molecular analysis methods. Consequently, total 15 virulent isolates, 3 isolates from each province were sequenced for 5′ and 3′ ends of ITS of rDNA and nBLAST of the ITS sequences revealed that Zymoseptoria tritici was the agent of Septoria leaf blotch. The highest disease prevalence () was recorded at Adana and followed by Osmaniye with , Kahramanmaraş (), Mersin (), and Hatay (). Key words: ITS, .