tailieunhanh - Genetic and phenotypic variation among Turkish terrestrial orchid species as revealed by RAPD and morphological characteristics

Terrestrial orchid species are natural sources of salep and a closely related group of plant species widely distributed throughout Turkey. The phylogenetic relationship among fourteen different tuber-producing orchid species was investigated after analyzing phenotypic and genetic variation within and among the natural population through fifteen morphometric traits and ten random amplified polymorphic DNA (RAPD) primer combinations. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2018) 42: 227-236 © TÜBİTAK doi: Research Article Genetic and phenotypic variation among Turkish terrestrial orchid species as revealed by RAPD and morphological characteristics 1 2 2 2, Gülden SANDAL ERZURUMLU , Nusrat SULTANA , Mehtap VURAL , Sedat SERÇE * Department of Landscape Architecture, Faculty of Architecture, Niğde Ömer Halisdemir University, Niğde, Turkey 2 Department of Agricultural Genetic Engineering, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, Niğde, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: Terrestrial orchid species are natural sources of salep and a closely related group of plant species widely distributed throughout Turkey. The phylogenetic relationship among fourteen different tuber-producing orchid species was investigated after analyzing phenotypic and genetic variation within and among the natural population through fifteen morphometric traits and ten random amplified polymorphic DNA (RAPD) primer combinations. Statistical analyses (principal component analysis (PCA), principal coordinate analysis (PCoA), and cluster analysis) using the generated data identified taxonomic and genetic distance within the studied plant samples. The results of PCA from morphological traits show that there are no major groupings within and among different species instead somehow overlapping with few distinctly characterized species. In addition, the UPGMA-based phenogram with Euclidean distance (0–1) produces five major clusters among the studied orchid species according to their taxonomic status with few exceptions. On the other hand, PCoA and the phylogenetic dendrogram with the coefficient (–) from RAPD band profiles determine the true genetic diversity of those species. Although both combinations .

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