tailieunhanh - Differential oxidative response to fluoropyrimidines in colorectal cancer cell lines
Colorectal cancer (CRC) is the second most common human malignancy in women and the third in men, accounting for approximately 8% of cancer-related deaths and million newly diagnosed cases annually worldwide. | Turkish Journal of Biology Turk J Biol (2017) 41: 187-194 © TÜBİTAK doi: Research Article Differential oxidative response to fluoropyrimidines in colorectal cancer cell lines 1,2, 1 Ufuk ÖZER *, Karen WOOD BARBOUR Department of Biological Sciences, Faculty of Science, University of South Carolina, Columbia, SC, USA 2 Department of Molecular Biology and Genetics, Faculty of Science, Dicle University, Diyarbakır, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: Colorectal cancer (CRC) is the second most common human malignancy in women and the third in men, accounting for approximately 8% of cancer-related deaths and million newly diagnosed cases annually worldwide. CRC cell lines differ in morphological features, growth rate, and their genetic alterations, implicating the extent of variability among the lines. The goal of the current study was to investigate the phenotypes of CRC lines related to NADPH oxidase (NOX) activity and expression of NOX2 subunits in response to fluoropyrimidines. NOX activity in response to 5’-fluoro-2’-deoxyuridine (FdUrd) and VAS2780 was measured in CRC cell lines. Cells were treated with FdUrd in order to determine apoptotic cell death and mRNA expressions of NOX2 cytoplasmic subunits. We find that both basal and drug-induced NOX activity differ in the lines. Similarly, drug-mediated apoptotic indices vary in the cell lines at basal levels. Among NOX1 and NOX2 subunits, only NOX2 accessory subunits, p67phox, p40phox, and p47phox, differ in the lines. Our results show that CRC cells have diverse oxidative background and their oxidative response to anticancer drugs vary, while they all exhibit increases in NOX activity. Key words: NADPH oxidase, fluoropyrimidines, oxidative stress, CRC cells 1. Introduction Colorectal cancer (CRC) is the third most common cancer worldwide in males and the disease rate is .
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