tailieunhanh - Identification of proteins differentially accumulated in Enterococcus faecalis under acrylamide exposure

Analysis of bacterial proteomes can be used to obtain large amounts of information about adaptive microbial mechanisms to changing extracellular conditions. In the past, many bacterial species with the ability to degrade acrylamide were isolated. In this study differences in the Enterococcus faecalis proteome upon acrylamide exposure were investigated. | Turkish Journal of Biology Turk J Biol (2017) 41: 166-177 © TÜBİTAK doi: Research Article Identification of proteins differentially accumulated in Enterococcus faecalis under acrylamide exposure Aleksandra BOCIAN*, Konrad HUS, Marcin JAROMIN, Mirosław TYRKA, Andrzej ŁYSKOWSKI Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, RzeszÓw, Poland Received: Accepted/Published Online: Final Version: Abstract: Analysis of bacterial proteomes can be used to obtain large amounts of information about adaptive microbial mechanisms to changing extracellular conditions. In the past, many bacterial species with the ability to degrade acrylamide were isolated. In this study differences in the Enterococcus faecalis proteome upon acrylamide exposure were investigated. We revealed substantial changes in the proteome of bacteria cultured in different environmental conditions. Microorganisms exposed to acrylamide showed higher accumulation of proteins associated with energy metabolism and its regulation. Moreover, several proteins involved in protection of cells from stress conditions were also identified. These biomacromolecules are involved in proper folding of newly synthesized polypeptides like chaperones or participate in mechanisms of DNA repair. In contradiction with previous reports, the presence of amidase was not detected. However, identification of aminopeptidase with activity to hydrolyze amino acid amides can indicate that it can degrade acrylamide to acrylic acid and ammonia instead of amidase. According to identified proteomic profiles, a new mechanism of acrylamide degradation by Enterococcus faecalis is proposed. Key words: Enterococcus faecalis, acrylamide, biodegradation, proteomics, genome sequencing 1. Introduction The presence of acrylamide at high concentrations in the environment is very dangerous due to its .

• Sinh học
• Enterococcus faecalis
• Microbial mechanisms
• Genome sequencing
• Acrylamide monomer
• Bioinformatic analysis
• Virulence genes
• Identification and antimicrobial resistance
• Antimicrobial resistance
• Enterococcus faecalis isolated
• Diabetic foot patients
• Chlorhexidine gluconate
• Mueller hinton agar
• Enterococcus faecalis (In vitro study)
• Chlorhexidine gluconate root canal irrigant
• Multiple antibiotic resistance
• 16s rRNA amplification and sequencing
• Diseased tilapia
• Antibiotic resistance of enterococcus faecalis
• Scientific reports
• biochemistry
• molecular Biology
• decarboxylase
• oxaloacetate
• Virulence gene
• Antibiotic resistance
• Human milk
• Polymerase chain reaction
• Tạp chí Y học
• Bài viết về y học
• Dịch chiết lá Neem
• Sodium hypochlorite
• Dung dịch bơm rửa ống tủy
• Da liễu học
• Bệnh viện Da liễu trung ương
• Nồng độ diệt khuẩn tối thiểu
• Cầu khuẩn Gram dương
• BMC Cancer
• Bacteroides fragilis
• Colorectal cancer
• Fusobacterium spp
• Streptococcus gallolyticus
• Nghiên cứu y học
• Đặc điểm kháng kháng sinh
• Enterococcus faecalis kháng fluoroquinolone
• Nhiễm trùng đường tiết niệu
• Nhiễm khuẩn huyết
• BMC Pediatrics
• Lung abscess
• Community acquired pneumonia
• Oral amoxicillin
• Tinh dầu tràm trà Úc
• Tinh dầu hương nhu trắng
• Nhiễm trùng ống tủy
• Enterococcus kháng vancomycin
• Kháng kháng sinh của Entrococcus faecalis
• Viêm nội tâm mạc
• Enterococcus spp
• E faecalis
• E faecium
• High level aminoglycoside resistance
• Tertiary health care hospital in Agra
• Report medicine
• traditional medicine
• immune system
• Antimicrobial
• biosynthesis
• công nghệ sinh học
• kỹ thuật di truyền
• nghiên cứu khoa học
• chọn tạo giống
• vi sinh vật
• biến đổi gene
• Antimicrobial activity
• Candida albicans
• Orthodontic arch wires
• Streptococcus mutans
• Antimicrobial compound
• Burkholderia paludis
• The role of reactive oxygen species
• BMC Pharmacology and Toxicology
• Bacterial isolates
• Staphylococcus aureus
• Neisseria gonorrhea
• Diabetic foot ulcer
• Diabetic foot ulcer infections
• Major complication of diabetes mellitus
• Punica granatum
• Punica granatum extracts
• Journal of Chemistry
• L methionine
• Microwave assisted synthesis
• Listeria monocytogenes
• Macro algae
• Algal extract
• Escherichia coli
• Candida sp
• Nhiễm trùng nặng sau mổ ghép gan
• Nhiễm trùng đường mật do sỏi mật
• Hẹp đường mật sau mổ ghép gan
• Characteristics of Tacca Leontopetaloides
• Tacca Leontopetaloides L Kuntze
• Constituents of leaves
• Antioxidant and antimicrobial activities