tailieunhanh - Assessment of the function and expression pattern of auxin response factor B3 in the model legume plant Medicago truncatula

The phytohormone auxin is a critical signal molecule, regulating fundamental processes in plant growth and development, such as shaping the root and shoot architecture, organ patterning, and nodulation. | Turkish Journal of Biology Turk J Biol (2017) 41: 66-76 © TÜBİTAK doi: Research Article Assessment of the function and expression pattern of auxin response factor B3 in the model legume plant Medicago truncatula 1, 2 2 3,4 Miglena REVALSKA *, Valya VASSILEVA , Grigor ZEHIROV , Sofie GOORMACHTIG , Anelia IANTCHEVA 1 AgroBioInstitute, Sofia, Bulgaria 2 Institute of Plant Physiology and Genetics, Bulgarian Academy of Science, Sofia, Bulgaria 3 Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, Ghent, Belgium 4 Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium Received: Accepted/Published Online: 1 Final Version: Abstract: The phytohormone auxin is a critical signal molecule, regulating fundamental processes in plant growth and development, such as shaping the root and shoot architecture, organ patterning, and nodulation. Auxin regulates plant gene expression mainly through auxin response factors (ARFs), which bind to auxin response elements in the promoter, upstream of auxin-activated genes. Here we examine and assess the function and expression pattern of a gene described as an auxin response factor, containing a DNAbinding pseudobarrel and B3 DNA-binding domains, from Medicago truncatula (МtARF-B3). For the model legume species M. truncatula, stable transgenic plants with MtARF-B3 overexpression, downregulation, and transcriptional reporters were constructed. Phenotypic and morphological evaluation of the obtained transgenic plants confirmed the important role of MtARF-B3 in general plant growth and development, modeling of root architecture, and development of seeds. Detailed histochemical and transcriptional analysis revealed expression of the gene in various stages of somatic embryogenesis, during formation of plant organs and tissues, and symbiotic nodulation. The fact that MtARF-B3 was strongly .

• Sinh học
• Auxin response factor B3
• Model legume
• Gene expression
• Plant growth
• Plant development
• Organ patterning