tailieunhanh - Enzymatic hydrolysis of coconut oil using free and immobilized porcine pancreas lipases

The aim of this study is to evaluate the effect of some factors on the hydrolysis of coconut oil (CO) in the present of two kind of enzymes, the free lipases and immobilized lipases porcine pancreas. The activities of these two lipases under the optimal hydrolysis conditions was determined. | SCIENCE & TECHNOLOGY DEVELOPMENT, , - 2016 Enzymatic hydrolysis of coconut oil using free and immobilized porcine pancreas lipases Nguyen Thi Ai Van 1 Phan Ngoc Hoa 2 Tran Bich Lam 2 Chau Tran Diem Ai 2 1 2 Industrial University of HCMC Young Researchers Association Ho Chi Minh city University of Technology, VNU-HCM (Manuscript Received on November 24th, 2015, Manuscript Revised May 11th, 2016) ABSTRACT The aim of this study is to evaluate the effect of some factors on the hydrolysis of coconut oil (CO) in the present of two kind of enzymes, the free lipases and immobilized lipases porcine pancreas. The activities of these two lipases under the optimal hydrolysis conditions was determined. of the enzyme to substrate of 90(U/mL), and the pH condition of at the temperature of 40oC. The best reaction condition the case of using immobilized porcine pancreas lipase as the catalyst was determined, including: the ratio enzyme to substrate of 393U/g, the pH condition of and the temperature of 35oC. The hydrolysis degree of CO by immobilized porcine pancreas lipase was increased slower than free lipase at the first time. The highest hydrolysis degree achieved with immobilized porcine pancreas and free porcine pancreas lipase was and , respectively. The effects of factors on hydrolysis degree of coconut oil was investigated: the ratio of enzyme to substrate, the pH condition, and the temperature. The best conditions for the high hydrolysis degree in case of using lipase from porcine pancreas ascatalyst included: the ratio Keywords: coconut oil, hydrolysis, immobilized porcine pancreas lipase, free porcine pancreas lipase 1. INTRODUCTION Coconut oil (CO) is a popular agricultural product in Vietnam as well as in other tropical CO is composed of short and medium chain fatty acids. Coconut oil has been used for health countries such as Philippines, Thailand, India, Indonesia, Sri Lanka, Malaysia and New .