tailieunhanh - Cloning and expression of the dengue virus non structural protein 1 (NS1) in E. coli

The isolated ns1 gene was high identity with Dengue virus 1 polyprotein-like gene (gene ID: ). The ns1 gene was successfully inserted into the expression vector pET22b(+) in an open reading frame. The successful expression of recombinant NS1 protein (rNS1) in E. coli BL21 (DE3) was checked by SDS-PAGE and confirmed by Western blot. Some suitable conditions for high expression of rNS1 protein were mM IPTG, 4 hours induction and initial inoculum density of . | Journal of Science & Technology 126 (2018) 038-043 Cloning and Expression of the Dengue virus Non-structural Protein 1 (NS1) in E. coli Truong Quoc Phong*, Chu Thi Minh Hai, Le Van Hoang, Dang Thi Huyen Trang Hanoi University of Science and Technology – No. 1, Dai Co Viet Str., Hai Ba Trung, Ha Noi, Viet Nam Received: July 26, 2017; Accepted: June 25, 2018 Abstract Dengue is an infectious disease that poses a threat to approximately billion people in 128 countries in the world. A report in 2013 indicates average of 390 million dengue infections per year. Non-structural protein 1 (NS1) is a highly conversed dengue virus glycoprotein and considered as a specific biomarker for serodiagnosis of dengue infection. In the present study, the ns1 gene encoding the non-structural protein 1 (NS1) from the Vietnam Dengue virus isolate was cloned and sequenced. The isolated ns1 gene was high identity with Dengue virus 1 polyprotein-like gene (gene ID: ). The ns1 gene was successfully inserted into the expression vector pET22b(+) in an open reading frame. The successful expression of recombinant NS1 protein (rNS1) in E. coli BL21 (DE3) was checked by SDS-PAGE and confirmed by Western blot. Some suitable conditions for high expression of rNS1 protein were mM IPTG, 4 hours induction and initial inoculum density of . Keywords: Dengue virus, E. coli, non-structural protein (NS1), recombinant 1. Introduction the current antibody test in case dengue antibodies are still undetectable [4]. * Dengue virus is one of the most dangerous viruses, posing a threat to one-third of the global human population in tropical and sub-tropical regions. Dengue virus is the cause of Dengue Fever, Dengue Hemorrhagic Fever (DHF) and most seriously Dengue shock syndrome (DSS) [1]. Dengue is caused by one to four dengue serotypes (DENV14). In Vietnam, DENV1 is the highest prevalence among four serotypes. Dengue is a single, positivesense, RNA virus with a genome of approximately .

• Sinh học
• Journal of Science and Technology
• Cloning and expression
• The dengue virus non structural protein 1 in E
• coli
• Dengue virus 1 polyprotein like gene
• High expression of rNS1 protein
• Journal of Marine science and Technology
• Marine science and Technology
• Marine science and Technology no 56
• Marine science and Technology year 2018
• Evaluation method
• Electrical energy management model
• Marine science and Technology no 53
• Diesel engines
• Journal of Fisheries science and Technology
• Fisheries science and Technology
• Fisheries science and Technology no 3
• Fisheries science and Technology year 2016
• Striped catfish
• Common carp cyprinus carpio
• Fisheries science and Technology no 4
• Fisheries science and Technology year 2018
• Coastal fi shing activities
• White striped cleaner shrimp
• Journal of Science
• Journal of Technology
• Technology Management
• Science and Technology
• International integration
• Science and Technology Strategy
• Evaluation of science and technology
• Global journal of computer science
• Global journal of computer science and technology
• Information & technology
• GIS based fire emergency response system
• Marine science and technology no 61
• Actual GPS receivers
• Display information on stability
• Nuclear science and technology
• The journal of Nuclear science and technology
• Rotary rack served for elemental concentration
• Seawaterusing co precipitation method
• Natural radionuclides
• Experimental data
• Low energy experiments
• Multigroup cross sections
• Stable isotopic values
• Radial blanket
• Dissolved nitrogen compounds
• Irradiation channels
• Neutron rich nuclei
• Prescila neutron probe
• Full core conversion
• Multipurpose research reactor
• Aquatic environment
• Geochemical parameters
• External reactor vessel cooling method
• Radiation crosslinked scaffolds
• Adipose derived stem cell culture
• Treatment plans
• Minor actinide compositions
• IM NAA of non standard geometrical samples
• Cross shaped obstacle
• University entrepreneurship
• Entrepreneurial research university
• Technology innovation
• Industry university cooperation
• Regional innovation
• Journal of technology and science education
• Bring science and technology closer to secondary school
• Technology closer to secondary school
• Secondary school teachers
• Face to face
• Technology development
• Technology commercialization
• The role of informal science centers in science education
• Informal science center
• Attudes toward science
• Scientfic thinking skills
• Scientific research
• Research quality
• Strategy and policy
• Research Organizations
• Policy Planning
• Science and Technology development
• Theory Z
• Human Resources
• Development policy of Japan
• ‘Standards’ on the bench
• Technological literacy render an adequate image of technology
• Adequate image of technology
• Standards for technological literacy
• Philosophy of technology
• The nature of technology
• Concepts of technology
• Post secondary project based learning in science
• Post secondary project based learning in technology
• Post secondary project based learning in engineering and mathematics
• Post secondary project