tailieunhanh - Development of a lateral flow immunoassay test strip for the rapid detection of rubella virus

This study was designed to construct a lateral flow immunoassay test strip for rapid detection of rubella virus. Rabbit anti-rubella antibody and pig anti-rubella antibody were successfully purified from sera of rabbit and pig infected with rubella virus. The yields of IgG affinity purification were mg/ml and mg/ml serum, respectively. | Journal of Science & Technology 126 (2018) 032-037 Development of a Lateral Flow Immunoassay Test Strip for the Rapid Detection of Rubella Virus Truong Quoc Phong1*, Nguyen Thi Anh Hoa1, Ngo Thu Huong2, Pham Huong Giang1, Khuat Huu Thanh1 1 School of Biotechnology and Food Technology, Hanoi University of Science and Technology 2 Center for Research and Production of Vaccines and Biologicals Received: August 16, 2017; Accepted: June 25, 2018 Abstract This study was designed to construct a lateral flow immunoassay test strip for rapid detection of rubella virus. Rabbit anti-rubella antibody and pig anti-rubella antibody were successfully purified from sera of rabbit and pig infected with rubella virus. The yields of IgG affinity purification were mg/ml and mg/ml serum, respectively. Suitable conditions for conjugation of antibody and gold nanoparticles (AuNPs) were determined: µg rabbit anti-rubella antibody was conjugated with 10 µl of AuNPs at pH 10, 37oC for 90 min. Similarly, suitable conditions for immobilization of pig anti-rubella antibody on the nitrocellulose were 3 µg of antibody, nitrocellulose membrane of Pall Vivid 170, immobilizing buffer of 20 mM sodium borate plus 2% sucrose, at 37oC for 30 min. The complete test strip was successfully constructed for rapid detection of rubella virus. Keywords: Rubella virus, lateral flow immunoassay, rapid detection 1. Introduction the virus to form surface spikes. E1 protein is the dominant surface molecule and associated with neutralizing and haemagglutinating epitopes [4]. * Rubella is an infectious disease caused by rubella virus and has a worldwide distribution [1]. Rubella disease usually expresses mild symptoms including a low-grade fever, sore throat and a rash with starting on the face and spreading to the rest of the body. If a woman is infected by rubella virus while she is pregnant, rubella virus can transmitted through the placenta to the fetus. Rubella can cause a miscarriage or .