tailieunhanh - Antimicrobial properties of silene multifida (Adams) Rohrb. plant extracts

Specimens of Silene multifida (Adams) Rohrb. were collected in the vicinity of Yomra, Trabzon, Turkey. The fresh plants were air-dried and chopped. This material was extracted with cold chloroform (CHCl3). The chloroform extract was dissolved in dimethyl sulphoxide (DMSO), and, following column chromatography, 6 fractions were obtained. | Turk J Biol 30 (2006) 17-21 © TÜB‹TAK Antimicrobial Properties of Silene multifida (Adams) Rohrb. Plant Extracts Ömer ERTÜRK1, Hatice KATI2, Nurettin YAYLI3, Zihni DEM‹RBA⁄2 1 Department of Biology, Faculty of Arts and Sciences, Ondokuz May›s University, 52750 Perflembe, Ordu - TURKEY 2 Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, 61080 Trabzon - TURKEY 3 Department of Chemistry, Faculty of Arts and Sciences, Karadeniz Technical University, 61080 Trabzon - TURKEY Received: Abstract: Specimens of Silene multifida (Adams) Rohrb. were collected in the vicinity of Yomra, Trabzon, Turkey. The fresh plants were air-dried and chopped. This material was extracted with cold chloroform (CHCl3). The chloroform extract was dissolved in dimethyl sulphoxide (DMSO), and, following column chromatography, 6 fractions were obtained. The serial dilutions (, 1, 2, 4 and 10 mg/ml) of these fractions were prepared in 5% Tween 20. The fractions were tested for antimicrobial activity using the agar diffusion technique against 6 bacteria, Bacillus subtilis, Staphylococcus aureus, Esherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae and Proteus vulgaris, and 1 fungus, Candida albicans. Fractions 1, 2, 3, 4, 5 and 6 of the extract of S. multifida showed activity against all tested bacteria. Fractions 1 and 5 showed higher antifungal activity than fractions 2 and 4. On the other hand, fractions 3 and 6 did not show any antifungal activity. Key Words: Silene multifida, Plant extract, Antimicrobial activity Silene multifida (Adams) Rohrb. Bitki Ekstraktlar›n›n Antimikrobiyal Özellikleri Özet: Silene multifida (Adams) Rohrb. bitkisine ait numuneler Trabzon’un Yomra ilçesi civar›ndan topland›. Taze bitkiler aç›k havada kurutuldu ve parçaland›. Bu materyaller so¤uk kloroformla ekstre edildi. Kloroformun çeflitli fraksiyonlar› Dimetil sülfoksit (DMSO) ile çözüldü ve 6 fraksiyon elde edildi. Fraksiyonlar›n seri dilisyonlar› (10,