tailieunhanh - Tính chất hệ enzym tiêu hóa trong các cơ quan nội tạng cá tra (Pangasius)

Trong ngành chế biến cá, nội tạng được xem là phế phẩm và thường được loại bỏ. Mục tiêu của nghiên cứu là tận dụng nội tạng cá Tra (Pangasius), chiếm 5-6% trọng lượng cơ thể cá, để thu nhận các enzym tiêu hóa. Nội dung nghiên cứu tính chất của các enzym lipase, protease, amylase trong các cơ quan nội tạng khác nhau của cá Tra. Kết quả cho thấy trong ba nhóm nguyên liệu: gan tụy, ruột, nội tạng hỗn hợp thì gan tụy là nguồn nguyên liệu tốt nhất để thu nhận cả ba loại enzym tiêu hóa. | Science & Technology Development, Vol 14, 2011 PROPERTIES OF DIGESTIVE ENZYMES FROM VISCERAL ORGANS OF TRA (PANGASIUS) CATFISH Vuong Bao Thy (1), Tran Bich Lam(2), Luu Duan(3) (1) Cuu Long University (2) University of Technology, VNU-HCM (3) Saigon Technology University (Manuscript Received on May 13rd 2010, Manuscript Revised October 14rd 2011) ABSTRACT: In fish processing, viscera are generally considered waste products and often discarded. Our research objective is to use Tra (pangasius) catfish viscera which accounts for 5-6% of body weight as a source of digestive enzymes. This paper investigated the influence of individual visceral organs of Tra catfish on the activities and properties of lipase, protease and amylase. Results revealed that the hepatopancreas was the best source for three digestive enzymes, with the highest activities of lipase ( protein), protease ( protein) and amylase ( protein), in comparison with intestine or mixed viscera. The optimal pH of Lipases from the hepatopancreas, intestine and mixed viscera were of 8, and 8 respectively, of Proteases were of , , 9, and of Amylases were of , 8 and 8, respectively. The maximum activities of lipase, protease, and amylase were found at the temperature of 50, 55 and 400C, respectively. Keywords: digestive enzymes, viscera, lipase, protease, amylase. less heat treatment, when desired in a given 1. INTRODUCTION process. Enzymes from cold-adapted fish species In Tra (pangasius) catfish industry, 30% often have higher catalytic activities at low are fillets, the rest are by-product and waste, in temperatures than their counterparts from which, fish viscera, accounting for 5-6% of warm-blooded of total mass, has wide biotechnological potential enzymes at low temperatures may be of interest as a source of digestive enzymes. Hence, the for several industrial applications of enzymes, objective of this study was to study on the such .