tailieunhanh - The effectiveness of silibinin on the change in some blood biochemical indices and liver histopathology of rabbit experimentally poisoned with amanita virosa mushroom

This study was conducted to investigate the role of silibinin in the change of biochemical indices of rabbits poisoned with of amanita virosa to provide the experimental evidence on the ability of hepatocellular protection of silibinin against toxic effect of amatoxin. | Journal of military pharmaco-medicine n06-2018 THE EFFECTIVENESS OF SILIBININ ON THE CHANGE IN SOME BLOOD BIOCHEMICAL INDICES AND LIVER HISTOPATHOLOGY OF RABBIT EXPERIMENTALLY POISONED WITH AMANITA VIROSA MUSHROOM Ngo Thi Thanh Hai*; Nguyen Thanh Binh*; Tran Van Tung*; Be Hong Thu** SUMMARY Objectives: To evaluate the effectiveness of silibinin on the change in some blood biochemical indices and liver histopathology of rabbit poisoned with Amanita virosa mushroom in order to get evidence of protective effect of the drug against the toxicity of amatoxin. Subjects and methods: Rabbits were randomly grouped then poisoned with Amanita virosa mushroom. 12 hour after the intoxication, the rabbits were given silibinin then blood samples were collected for the testing of GOT, GPT, GGT, urea, glucose, total bilirubin and direct bilirubin on a automated system. Liver histology was processed and examined followed standard HE staining procedure. Results: GOT, GPT, GGT concentrations in blood of rabbits poisoned with Amanita rd th virosa in the group with silibinin treatment decreased on the 3 and 5 days after poisoning compared to the group without treatment. Silibinin treatment also decreased hepatocellular damages caused by Amanita virosa mushroom poisoning. Conclusion: Silibinin has protective effect against the toxicity of Amanita virosa mushroom. * Keywords: Silibinin; Amatoxin; Amanita virosa; Blood biochemical indices; Liver histopathology; Rabbit. INTRODUCTION According to statistics from the Poison Control Center of Vietnam Military Medical University from 2004 to 2011, in Backan province there were 28 incidences of mushroom poisoning with the total number of 94 infected people and 14 deaths. The results of investigation showed that the deaths from poisoning of poisonous mushrooms in Backan province were caused by Amanita virosa [1]. Amanita virosa is pure white, fleshy, very beautiful and attractive. Amanita virosa contains amanitines that cause slow and .